The positive clones were sequenced, and the sequences were analyzed by the BLAST program for homology search.
挑选阳性克隆进行测序并用BLAST软件进行同源性分析。
Three rounds of biopanning were carried out. The positive clones were randomly selected, detected and sequenced.
随机挑取噬菌体克隆,检测其特异性并进行序列分析;
The results showed that the positive clones screened by G418 were mouse fibroblast cells with high expression level of VEGF.
结果显示:经g 418筛选后的阳性细胞克隆,为高表达VEGF的鼠成纤维细胞模型。
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