Results 100 % of frozen tissues and 24.1% of paraffin-embedded tissues could get either methylated production or unmethylated production.

结果100%的新鲜组织和24.1%的石蜡包埋组织至少可以得到甲基化和非甲基化产物中的一种产物。

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It relies upon the selective deamination of unmethylated cytosine to uracil after treatment with sodium bisulphite, usually followed by PCR amplification of the chosen target region.

它依赖于用 亚硫酸氢钠处理后，非甲基化的 胞嘧啶选择性的去氨基为 尿嘧啶，通常接着所选的靶向区域的PCR扩增。

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Bisulphite conversion of unmethylated cytosine residues followed by detection methods such as sequencing of distinct loci has become accepted as the gold standard for detecting 5-methylcytosines.

非甲基化胞嘧啶残基的亚硫酸氢盐转换，接着是检测方法，例如不同位点的测序已经被接受为检测5甲基胞嘧啶的金标准。

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In some cases that backbone gets methylated and those regions of the DNA that are methylated get treated differently by the cell than unmethylated regions.

一些情况下,主链会甲基化,该甲基化区域的DNA,和未甲基化区域的DNA,会被细胞区别对待

耶鲁公开课 - 生物医学工程探索课程节选

Cells have ways to repair such mutations, but in this case, when the cell repairs the DNA, it is left unmethylated and the cell's memory of the gene's 'off' status is lost.

BBC: NEWS | Health | Poor cell memory is key to cancer