重组痘苗病毒载体在消化道肿瘤基因治疗中的应用 热点之一。基因转移是基因治疗的关健和基础,寻找高效、安全的载体表达系统是基因治疗研究中的重点和难点。痘苗病毒(vaccinia virus,VV)作为基因治疗的载体系统之一正越来越受到人们重视[1],已成为继逆转录病毒和腺病毒之后作为基因治疗载体
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The researchers began by removing two genes from a vaccinia virus that are necessary for its growth in normal cells.
研究人员们首先将痘苗病毒中在正常细胞中生长所必须的两个基因去除。
Objective To investigate expression of IL 6 in non replicating vaccinia virus and its immune effects on recombinant virus.
目的研究白细胞介素6 (IL 6)在非复制型痘苗病毒中的表达及其对重组痘苗病毒免疫效果的影响。
The detection methods for vaccinia virus were developed and provide a base for laboratory diagnosis of variola-related viruses.
所建立的痘苗病毒系列检测方法,为天花相关病毒的实验室诊断奠定了基础。
And so in the 1940s, a group of people led by Collier started to develop a large scale production method for making a reliable source of vaccinia virus.
在二十世纪四十年代,科利尔带领一群人开始研究,一种大规模的生产方法,这种方法能制得牛痘病毒的可靠来源
We know now that it's caused by a related virus, called vaccinia, remember the virus that causes smallpox is called variola.
我们现在知道这病由一种相关的病毒引起,这种病毒叫牛痘病毒,想想天花病毒也被叫做牛痘病毒
So, how could you produce--the question is, how could you produce large quantities of this- of this microorganism, this virus vaccinia under controlled conditions?
问题是该怎么进行生产,你怎么大批量生产这种微生物,即在可控条件下生产牛痘病毒
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