050401摘要 关键词：重组逆转录病毒；反义RNA；逆转录套式PCR；病毒效价 [gap=1338]Key words: recombinant retrovirus；antisense RNA；RT-nested PCR；viral titer
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Optimized parameters of electroporation were as follows: recombinant plasmid 20μg, electric capacity 975 μ F, voltage 220v, time 15ms. PA317 packaging cell line of consistant producing viron was isolated with generating viral titer 5.0 × 106-6.8× 106CFU.
电穿孔优化参数为重组质粒用量在20μg左右，电容975μF，电压220V，电击时间15ms时转染效率最高；筛选出了稳定产毒的PA317包转细胞系，病毒滴度在5.0×106—6.8×106CFU之间。
参考来源 - 软骨组织工程的种子细胞研究—人关节软骨细胞的永生化及其生物学特性·2,447,543篇论文数据，部分数据来源于NoteExpress
The viral titer was determined with the NIH3T3.
通过NIH3T3细胞测定病毒滴度。
For the local laboratory, it is better to use guinea pig erythrocytes to detect the viral HA titer.
基层实验室采用豚鼠血球更适合于病毒的HA滴度测定。
This experiment discussed the effect of yolk antibody on the passive immunity defense through preparing high-titer yolk antibody against the high-efficient rabbit's viral hemorrhage.
本试验通过制备高效价兔病毒性出血症高免卵黄抗体，探讨高免卵黄抗体对兔的被动免疫保护效果。
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