(3)Na+-K+泵电流测定:以全细胞膜片钳技术(whole-cell patch-clamp technique)记录Na+-K+泵电流(Ip),Na+-K+泵一次主动转运可将3个Na+泵出细胞外,同时泵进2个K+,因而可产生一个纯净的向外电流。
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whole-cell patch clamp technique 全细胞膜片钳技术 ; 膜片钳
whole cell patch clamp technique 全细胞膜片钳技术
whole cell patch-clamp technique 全细胞膜片箝技术
Whole-cell patch-clamp recording technique 膜片钳全细胞记录技术
whole-cell recording patch clamp technique 膜片钳全细胞技术
patch clamp whole-cell recording technique 膜片钳全细胞记录技术
patch-clamp whole cell recording technique 膜片钳全细胞记录技术
Method The experiment was carried out in freshly isolated bullfrog using a whole-cell patch-clamp technique.
方法实验采用的方法是全细胞膜片钳技术,实验材料为急性分离的牛蛙背根神经节神经元。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法传代得到单个HERG-HEK细胞,应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线(激活、失活、复活和去活化)。
Using the whole-cell patch-clamp technique, clear evidence was provided for the existence of a hitherto unreported, voltage-dependent nonselective cation current (NSCC) in rabbit ventricular myocytes.
应用全细胞膜片钳制技术,我们首次报道了在兔的心室肌细胞存在一种电压依赖性的非特异性阳离子流(NSCC)。
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