Evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using carboxyfluorescein diacetate succinimidyl ester labeling assay.
探讨应用活细胞荧光染料 CFSE 检测 T 淋巴细胞各亚群增殖反应的方法学。
Objective To evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using flow cytometry and carboxyfluorescein diacetate succinimidyl ester(CFSE) labeling assay.
目的探讨应用流式细胞术和活细胞荧光染料CFSE检测T淋巴细胞各亚群增殖反应的方法学。
Methods: Human peripheral blond lymphocyte (PBL) proliferation method was used and the population were classified by the length of service, classes of work and cumulated absorb dose.
方 法：用 外周血淋巴细胞细胞培养 法对放射工作人员进行微核检测并按工龄、工种、累积 吸收剂量等分类并设立对照组进行微核率的比较。
应用推荐