Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast (MEF) cells for isolation and culturing of embryonic stem (es) cells.

目的：建立ICR小鼠胚胎成纤维细胞饲养层培养体系，用于胚胎干细胞建系及研究。

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For utilization of the mouse embryonic stem (es) cells for various purposes, it is desirable that the cell lines are established from various sources such as inbred and outbred mouse strains.

为了充分利用小鼠胚胎干(es)细胞，就必须从众多小鼠品系中分离es细胞系。

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Objective To investigate the effects of developmental stages of tetraploid embryos on mouse chimeras from embryonic stem cells (ES).

目的 探讨四倍体胚胎发育阶段对胚胎干细胞（ES）嵌合体小鼠制备的影响。

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