2_临床医学论文 关键词】 咪唑啉受体;2-BFI;糖氧剥夺;脂质过氧化;大鼠 [gap=1133]Key words: imidazoline receptors;2-BFI;oxygen-glucose deprivation;lipid peroxidation;rats
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方法：采用原代培养的大鼠海马神经元经2h的氧糖剥夺(oxygen-glucose deprivation, OGD)和2h.4h、6h、12h、24h、48h的再灌注处理方法,建立神经元的缺血／再灌注的细胞模型。
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...结果表明，MT能减少DNA损伤，抑制线粒体释放细胞色素C和凋亡蛋白酶caspase-3的激活，阻止缺氧缺糖(oxygen-glucose deprivation，OGD)缺失引起的线粒体膜电位改变，说明在缺血再灌注中MT通过线粒体途径调节凋亡。
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方法：采用原代培养的大鼠海马神经元经2h的氧糖剥夺(oxygen-glucose deprivation, OGD)和2h.4h、6h、12h、24h、48h的再灌注处理方法,建立神经元的缺血／再灌注的细胞模型。
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oxygen-glucose deprivation injury 缺氧无糖损伤
Oxygen-glucose deprivation model 缺氧缺糖模型
Transient oxygen-glucose deprivation 短时程糖氧剥夺
model of oxygen-glucose deprivation 缺糖缺氧模型
Oxygen- glucose deprivation and reintroduction 复糖复氧
oxygen and glucose deprivation 缺氧无糖损伤 ; 氧糖剥夺 ; 糖剥夺
Methods In vitro:hippocampal slices were prepared from 3-day-old neonatal rat pups and cultured using transwell plate for 4 days prior to experimentation. Tissues were randomly divided into normothermia(37℃)and hypothermia(33℃,24 h)groups after oxygen-glucose deprivation(OGD).
方法①体外实验:取3日龄大鼠海马脑片,培养至第4天用氧糖剥夺法制备标本,于33℃(亚低温组)和37℃培养48h(常温组);对照组脑片不进行氧糖剥夺处理,37℃培养7d。
参考来源 - 亚低温对缺氧缺血新生大鼠海马星形胶质细胞增殖和凋亡的影响Primary astrocyte was exposed to a paradigm of ischemic insult by using oxygen-glucose deprivation(OGD).
采用原代培养的新生大鼠皮层星形胶质细胞缺氧缺糖(oxygen-glucosedeprivation,OGD)方法,建立脑缺血的细胞模型。
参考来源 - 苦参碱抗大鼠局灶性缺血性脑损伤的作用及其保护星形胶质细胞的机制·2,447,543篇论文数据，部分数据来源于NoteExpress
Objective To establish oxygen-glucose deprivation model of rats′ cortex and hippocampusal neurons in vitro.
目的建立体外培养大鼠皮质及海马神经元缺氧缺糖模型。
Objective To understand the protective effect and possible mechanism of Lig on cultured rat astrocytes in oxygen-glucose deprivation (OGD) in vitro.
目的探讨川芎嗪对离体大鼠星形胶质细胞在糖氧剥离损伤中的作用及可能机制。
Methods An ischemic model was developed in PC12 cell line with treatment of oxygen glucose deprivation (OGD).
方法采用氧葡萄糖剥夺(ogd)方法在PC 12细胞上建立缺血模型。
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