This review focuses on the methods of isolation and cultivation of pathogenic free-living amoebae, including sample treatment, culture conditions, passage culture, pathogen detection, and maintenance.
本文综述该病原体国内外报道的分离培养方法，主要包括样品处理、培养条件、虫株传代、虫株致病性检测和虫株保存方法。
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