... 基因操作(Gene manipulation) 蛋白表达纯化(Protein expression and purification) 细胞培养(Cell culture) ...
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Objective: To construct the prokaryotic expression vector of human heat shock protein 70(HSP 70) and to induce the expression and purification of HSP 70 in vitro.
目的:构建人热休克蛋白70(HSP 70)的原核表达载体,诱导其表达并纯化。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
采用亲和层析和离子交换层析分离纯化,以细胞增殖实验测定表达蛋白的生物活性。
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