subtractive cDNA library construction 差减cDNA文库构建
forward subtractive cdna library 正向消减cdna文库
cDNA subtractive library cDNA消减文库
suppression subtractive hybridization cDNA library 抑制差减杂交cDNA文库
subtractive library of cdna 消减文库
cDNA selected-subtractive suppression library cDNA差减抑制杂交文库
With the exclusion of them, there are also some other functionally unknown transcripts were obtained from the subtractive cDNA library.
除此之外，从差减文库中还获得一些未知基因。
参考来源 - 琯溪蜜柚和度尾蜜柚自交不亲和性观察及相关基因克隆276 recombinant clones were obtained from forward subtractive cDNA library of meiosis period, 229 of which were positive insertional fragments with the average sizes of 100~1000 bp by PCR identification.
其中减数分裂时期正向消减文库共获得276个重组菌落,经菌液PCR鉴定获得了229个阳性插入片段,片段大小均在100～1000 bp之间。
参考来源 - 利用抑制消减杂交技术寻找无融合生殖相关基因·2,447,543篇论文数据，部分数据来源于NoteExpress
Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
目的：消减文库构建过程中，用P CR技术快速筛选重组阳性克隆。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
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