1.3.1 双向电泳(Two-dimensional polyacrylamide gel electrophoresis)12-13 1.3.2 液相色谱技术(Liquid chromatography)13
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Objective: To investigate the mechanism of plasticity after spinal cord injury, two-dimensional polyacrylamide gel electrophoresis (2-DE) technique was established through grading-up experiment condition.
目的：通过优化实验条件，建立双向电泳体系，初步获得正常大鼠脊髓蛋白质2—DE图谱。
参考来源 - 部分背根切断大鼠脊髓蛋白质组学研究·2,447,543篇论文数据，部分数据来源于NoteExpress
To establish a set of two-dimensional polyacrylamide gel electrophoresis (2-DE) techniques for proteomic research of hepatocellular carcinoma (HCC) tissues.
优化双向凝胶电泳的实验步骤，总结出一套适用于原发性肝癌组织的双向凝胶电泳技术。
Two dimensional polyacrylamide gel electrophoresis(2D PAGE) followed by mass spectrometry(MS) is the most widely used method of protein resolution and identification. But it exist shortcomings.
双相聚苯稀酰氨凝胶电泳（2 D PAGE）与质谱（MS）是分辨和鉴定蛋白的最常用的方法，但也存在有缺点。
Methods Using high resolution two dimensional polyacrylamide gel electrophoresis, the nuclear matrix proteins of 3 normal livers and 8 HCC were compared.
方法用双向电泳方法比较了3例正常肝和8例HCC核基质蛋白成分。
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