Objective To observe the changes of limbal stem cell morphology? Antigen and proliferative ability after primary culture in vitro.
目的探讨人角膜缘干细胞体外培养后细胞形态学、抗原性和增殖能力的变化。
Objective:To establish the quick and valid primary culture method of human placental villus in vitro and set up a human trophoblastic cell line with preliminary assessment.
目的：建立快速、有效的绒毛滋养细胞体外原代培养的方法以及建立人滋养细胞株和初步评价。
METHODS:Methods of in vitro cell culture model of human primary PDLC, tetrazolium bromide reduction (MTT) assay and direct contact were used.
方法：体外细胞培养，MTT （噻唑蓝）比色法，材料直接接触法。
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