方法:应用亚克隆技术将YCD和绿色荧光蛋白基因(green fluorescence protein,GFP)连接至慢病毒空载体pFUW,构建获得pGC-FU-YCD-GFP.
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方法：以绿色荧光蛋白(GFP)作为标记，观察hth_1基因在体外和脑内的表达。
Methods: By using GFP gene as a marker, to observe expression of human tyrosine hydroxylase type I (HTH1) gene in vitro or in brains.
本发明涉及生物技术中的基因工程领域，公开了一种含绿色荧光蛋白基因的植物转基因表达载体，以及其构建方法和应用。
The invention discloses a plant transgene expression carrier containing green fluorescent albumen gene, its method for constructing the same and use in genetic engineering field of biotechnology.
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