... 目的基因聚合酶链反应 Polymerse Chin Rection 等位基因聚合酶链反应 allelic specific PCR 基因的聚合 gene pyramiding ...
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目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
方法：以引物互为模板的聚合酶链反应合成目的基因片段。
Methods: The PCR technique in which the primers were used as template each other was adopted to obtain the foreign DNAs for Cloning.
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