...ADP水平降低时,KATP通道才被激活。通过进一步研究发现,糖酵解产生的ATP变化优先调节KATP通道活性。糖酵解酶3-磷酸甘油醛脱氢酶(GAPDH)是KATP通道的辅助蛋白,该酶直接调节KATP通道的活性。
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(4)磷酸甘油醛脱氢酶(Phosphoglyceraldehyde Dehydrogenase)4 个亚基的蛋白质,活性中心有-SH每年亚基上连一个 NAD+(辅酶)甘油醛的 H+传给 NAD+二、三羧酸循环(TCA cycle)也叫柠檬酸环或 Kre...
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实时定量PCR分析脂肪酸合酶在口腔鳞癌中的表达-中国优秀硕士学位论文全文数据库 TC2000实时荧光定量基因扩增仪实施定量PCR扩增,以脂肪酸合酶(FAS)作为待测基因,葡萄糖3-磷酸甘油醛脱氢酶(gluceraldehyde phosphate dehydrogenase,GAPDH)作为内标基因以监测RT-PCR系统的稳定性,进行定量及系列稀释,建立标准曲线,确定实时RT-PCR的
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三磷酸甘油醛脱氢酶 GAPDH ; GAPD
3-磷酸甘油醛脱氢酶 GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ; Glyceraldehyde-3-phosphate
它们都用磷酸甘油醛脱氢酶基因的片段作为加载控制来进行改良。
They were modified with fragment of the glyceraldehyde phosphate dehydrogenase gene (GAPDH) as a loading control.
实验选用3-磷酸甘油醛脱氢酶基因(GAPDH)作为内参照,体外转录的RNA和阳性质粒作为两种标准品。
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was served as the internal reference, and RNA transcribed in vitro and positive plasmid were served as the standards in this experiment.
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