Viability/Cytotoxicity Assays(细胞存活率和细胞毒性检验试剂盒） Reporter Gene Assays(荧光素酶报告基因检测) qPCR(实时定量PCR试剂盒) ..
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瞬时转染人胚胎肾细胞293t，用荧光素酶报告基因检测这两种剪切形式的转录活性。
The transcriptional activity of the two splicing variants was detected with a luciferase reporter in transient transfections of human embryonic kidney293T cells.
以含雌激素反应元件的荧光素酶(ERE - LUC)为报告基因，通过检测荧光素酶活性来确定MDM2是否对ER有转录调节因子的作用。
The effect of MDM2 on the transcriptional activity of er was detected by the reporter gene containing estrogen responsive elements luciferase (ERE-LUC).
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