我们使用了依赖HCV IRES表达萤火虫荧光素酶的报告基因质粒。

We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.

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方法： 通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；

METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.

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