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Instead, they want to use somatic cell nuclear transfer, the same process used to clone Dolly the sheep in 1997, to create embryonic stem cells.
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If I understand the tetraploid complementation technique utilized in these experiments correctly, then, scientists would no longer need to generate embryos via somatic cell nuclear transfer (cloning).
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The problem is that making them using somatic-cell nuclear transfer involves breaking up viable embryos, since the cells in question are found inside early-stage embryos, called blastocysts.
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"Somatic cell nuclear transfer does not relate to cloning, and the people who call it therapeutic cloning are creating a lot of confusion, because it is not cloning at all, and it certainly is not reproductive cloning, " Specter said.
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Even Dolly the sheep, who was produced by cloning via somatic nuclear cell transfer, benefited from a male imprinted genome.
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Instead of generating embryonic stem cell lines via nuclear transfer, Hwang's group produced the stem cells from days-old embryos, a technique that had already been established by James Thomson at University of Wisconsin in 1998.
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In 2001, a gaur nicknamed Noah, a Southeast Asian threatened ox, was successfully created in the womb of Bessie, a cow, by scientists using a nuclear transfer process at Advanced Cell Technology in Massachusetts.
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Geron , the stem-cell company he founded before moving to ACT, has often talked of nuclear transfer as a way of figuring out how to make stem cells.
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"Nuclear transfer is really about the ability to take an adult cell and basically reprogram it so that you can grow an embryo from it, " Earp says.
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Only last year, she said, Egli and Paull and their colleagues demonstrated that the nuclear transfer of eggs cells could generate patient-specific stem cell lines for potential cell replacement therapy to treat diseases like diabetes.
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Geron (nadsaq: GERN - news - people ), the stem-cell company he founded before moving to ACT, has often talked of nuclear transfer as a way of figuring out how to make stem cells.
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