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Gajdosova will play lucky loser Lesia Tsurenko of Ukraine, who replaces Sharapova in the draw.
CNN: Injured Sharapova pulls out of season opening tournament
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He will be replaced in the draw by a lucky loser from one of the qualifying competitions.
CNN: U.S. No. 1 Isner out of Australian Open
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American "lucky loser" Blake Strode will take the Frenchman's place in Thursday's second-round clash with Bulgarian qualifier Dimitar Kutrovsky.
CNN: Monfils pulls out as Roddick battles through in San Jose
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Pavlyuchenkova ended the dream run of Lesia Tsurenko, a 116th-ranked Ukrainian who only made it into the main draw as a lucky loser from the qualifiers.
CNN: No. 1 Azarenka ruled out by foot problem
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Later on Saturday, world No. 1 Roger Federer begins his bid for a record-equaling 17th Masters 1000 tournament victory against Ecuador's Nicolas Lapentti, who made it into the main draw as a lucky loser in qualifying.
CNN: Champion Murray battered by Fish in another Miami upset
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By winning through to the second round, we put ourselves in with a chance of qualifying as a 'lucky loser' - meaning that if another team pulled out of the main draw, we would be in.
BBC: SPORT | Tennis | Road to Wimbledon part two
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On the men's side, No. 4-ranked David Ferrer needed five match points before clinching a 6-0, 7-5, 4-6, 6-3 win over American Tim Smyczek, who got into the main draw as a lucky loser from qualifying.
NPR: Djokovic, Sharapova Cruise At Aussie Open
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"I've never felt this fit, this strong, this happy to play -- and I think I can be even better, " fourth-ranked Williams told the WTA Tour's website after her 7-5 6-3 win over the 20-year-old Vandeweghe, who made it into the main draw as a lucky loser from qualifying.
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