Tomas Rosicky squandered the best chance for the Czechs during a first-half they totally dominated.
Substitute Tomas Rosicky saw a shot saved from Carson in a frantic finale.
The Czech Republic continued with their preferred 4-2-3-1 formation although an injury to Tomas Rosicky forced a change in personnel.
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Tomas Rosicky flashed a shot across goal and Nasri sent a drive just wide as Arsenal picked and probed at Villa.
Reina spilled the ball into his own net after Gunners striker Marouane Chamakh had bundled a Tomas Rosicky cross against the post.
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The England international skipped past a challenge after Tomas Rosicky's 13th minute pass, but Walcott lost his composure at the crucial moment.
The Frenchman revealed that midfielder Tomas Rosicky, who limped off injured in the second half, had "a little concussion from an accidental elbow".
Tomas Rosicky struck after the break for Arsenal but they were pegged back when Jonathan Tehoue scored for League One side Orient in the 89th minute.
Arsenal pulled a goal back with 18 minutes left, Gallas turning home from almost on the line after Kolo Toure touched on substitute Tomas Rosicky's corner.
Arsenal, who had Alex Song sent off for two yellow cards, missed a penalty when Tomas Rosicky blazed over after Ahmed Elmohamady had fouled Samir Nasri.
Campbell restored parity when he powered in a header from six yards after Tomas Rosicky had nodded back Thomas Vermaelen's flick from an inswinging Cesc Fabregas corner.
Van Persie and Tomas Rosicky have been out for most of the season, and when Eduardo also got injured there was little back-up for Arsenal's forward line.
Tomas Rosicky should have added a third, only to balloon the ball over the bar, while Fabregas went close with a shot after more good work by Henry.
But Arsene Wenger's young side kept their composure and pulled a goal back before halftime through Tomas Rosicky who lashed an angled shot past Bolton goalkeeper Jussi Jaaskelainen.
The visitors passed and probed and went close as Thomas Vermaelen headed wide from a Tomas Rosicky cross, before Reina tipped a Theo Walcott free-kick round the post.
Wilshere is not the only Arsenal player facing a fitness test on his return from international duty, with midfielders Andrey Arshavin and Tomas Rosicky also to be monitored.
Germany's Borussia Dortmund remain in second, three points behind the Gunners after goals from Jan Koller, Tomas Rosicky and Marcio Amoroso saw them win 3-1 at PSV Eindhoven .
Arsenal were being punished for their wastefulness in front of goal, and Chamakh was the culprit again with 10 minutes left as he headed substitute Tomas Rosicky's cross wide.
However, because of concerns over the fitness of fellow midfielder Tomas Rosicky, who has been troubled by a virus, Wenger elected to put Nasri in the starting XI against Huddersfield.
Although Johan Djourou suffered a hamstring injury in the 4-0 win over SC Neusiedl 1919 on Tuesday and Tomas Rosicky missed the match with a groin problem but Wenger has eased concerns over both players.
Just before the hour Aliadiere missed a good chance to score his first league goal since August 2002 when he sent the ball over the bar after good work by Justin Hoyte and Tomas Rosicky.
Walcott, Bendtner, Tomas Rosicky, Denilson and Laurent Koscielny have all recently returned from injury and they helped the visitors make a blistering start against a Newcastle line-up chosen with Sunday's Tyne-Wear derby against Sunderland in mind.
In the first-half the home team needlessly gave away possession far too often against a team of Arsenal's quality, while right-back Habib Beye repeatedly drifted too far inside and offered too much space to Tomas Rosicky.
However, for long periods Arsenal were the better side, but the Gunners rarely tested home keeper Petr Cech, who made two good saves from shots from Andrey Arshavin in the first half and substitute Tomas Rosicky late in the second.
Irish debutant Conor Henderson - another ball-playing midfielder - probed for a gap through the back-line and the 19-year-old's deflected pass was pounced on by Tomas Rosicky, who sped to the byeline to clip a square ball through the legs of Charlie Daniels across the box.
"It will be difficult and edgy until the end but the only thing I can say is that we are ready for a fight, " Wenger said after two goals from Tomas Rosicky put Arsenal ahead against eighth-placed West Brom before defender Per Mertesacker's sending-off led to a penalty by James Morrison.
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