检测两种干细胞端粒酶活性，结果证实它们具有克隆扩增的能力。
We identified telomerase activity in follicle dermal stem cells and marrow MSCs and demonstrated that they were capable of clonal expansion.
淋巴细胞在外周组织接触抗原而活化，克隆扩增的细胞浸润中枢神经系统。
Lymphocytes are primed in the peripheral tissues by antigens, and clonally expanded cells infiltrate the CNS.
目的探讨克隆聚合酶链反应(PCR)扩增产物的简便方法。
Objective To explore simple method for cloning the products amplified with polymerase chain reaction (PCR).
部分扩增产物t A克隆后测定核苷酸序列。
Partial amplification products were sequenced after T-A cloning.
肿瘤的产生是由于单个免疫细胞不受控制的大量扩增，产生大量的自身单克隆细胞。
The cancer starts when a single immune cell multiplies uncontrollably, producing many identical clones of itself.
方法：利用无血清培养和细胞克隆培养技术，从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞，进行体外扩增培养、传代、贴壁分化观察。
Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
方法设计特异的PCR引物，用RT-PCR技术分段扩增Q32株全长L、M片段，PCR产物纯化后直接测序，或用T-A克隆方法进行PCR产物克隆，然后进行遗传进化分析。
Methods The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.
一个突变细胞如发生克隆性扩增，就引起这些大量突变。
They arise when there is a clonal expansion of a mutated cell.
方法设计出针对各片段的特异性引物，用P CR方法从Z 37病毒感染的细胞提取细胞总rna，逆转录扩增、产物克隆t载体，纯化后测序，测定的序列应用DNASTAR软件比较分析。
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
该文以DNA PCR扩增的方法，从拟南芥基因组DNA中克隆出NPR1基因，通过序列分析，所克隆的NPR1 基因与报道的基因序列完全一致。
The NPR1 gene was amplified from Arabidopsis thaliana genome DNA by DNA-PCR method. The DNA sequenced analysis showed that the sequence of amplified NPR1 gene was the same as the published sequence.
利用通用引物（ITS1和ITS4）扩增、克隆和测序了大孢指疫霉ITS区段。
The internal transcribed spacer (ITS) region was amplified with the general primer (ITS1 and ITS4).
经设计通用引物、PCR扩增、克隆和测序，首次从分子水平鉴定了杂草赛葵上的双生病毒。
We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.
所构建的消减文库扩增后包含约1000个白色克隆。
结果筛选出3条在各克隆株扩增产物间有差异的引物。
Results 3 of the 23 single primers were found to be different between amplified products from different clonal strains.
方法将特异性引物从S8546病毒感染细胞PCR扩增的产物克隆于T载体，正确的克隆纯化后测序，应用DNASTAR软件比较分析。
Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
方法从Z 10株病毒感染的细胞提取总rna，将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定，应用dnastar软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR product was cloned into t vector, sequenced and analyzed using DNASTAR software.
方法利用RT -PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
利用DNA合成技术、DNA克隆技术、PCR扩增技术以及DNA芯片技术，结合密码学的计算复杂度理论，提出了一种基于DNA技术的加密方法。
Using the technologies of DNA synthesis, DNA clone, PCR and DNA chip and the theory of computational complexity, an encryption scheme is formed.
通过对随机挑选的23个片段进行克隆与测序，结果发现23个片段都是对应引物的RAPD扩增产物。
It was found these 23 fragments, which were cloned and sequenced after randomly selected, were the products from their corresponding RAPD primer amplification.
方法根据SV40- 776株设计合成三对引物对现有毒株进行PCR扩增并克隆测序。用这三对引物对56份猴肾、肺、脾及10批脊髓灰质炎疫苗进行检测。
MethodsKidneys, lungs and spleens of 56 monkeys and 10 poliovirus vaccines were examined by PCR using designed three pairs of primers on SV40-776 sequence.
将培养出肿瘤细胞克隆球给予传代扩增；
从轮状病毒G4型中扩增出vp7基因，将该基因构建于质粒并克隆培养。
Amplification VP7 gene from RV G4 serotype, construct a plasmid includes this gene and clone culture.
目的：对广西中药三七主要有效成分三萜皂甙合成关键酶法昵基焦磷酸合酶的基因进行体外扩增、克隆及序列分析。
Object: A cDNA encoding farnesyl pyrophasphate synthase (FPS) that is one of key enzymes in the synthesis of triterpene saponins from Panax notoginseng (Burkill) F. H. Chen ex C. Y. Wu & K. M.
目的：对广西中药三七主要有效成分三萜皂甙合成关键酶法昵基焦磷酸合酶的基因进行体外扩增、克隆及序列分析。
Object: A cDNA encoding farnesyl pyrophasphate synthase (FPS) that is one of key enzymes in the synthesis of triterpene saponins from Panax notoginseng (Burkill) F. H. Chen ex C. Y. Wu & K. M.
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