采用规范不变原子轨道GIAO法，计算它们的1H-NMR和13C-NMR化学位移值。
With the GIAO (gauge-independent atomic orbital) method, the 1H-NMR and 13C-NMR were also calculated theoretically.
方法样品经溶解、降解处理后，测定1h -NMR图谱，根据氢的化学位移和响应值，计算样品的脱乙酰度。
METHODS After being dissolved and depolymerized, the sample was determined by 1h-nmr. From the chemical shifts and the intensities of resonances of protons, the degree of deacetylation was determined.
方法样品经溶解、降解处理后，测定1h -NMR图谱，根据氢的化学位移和响应值，计算样品的脱乙酰度。
METHODS After being dissolved and depolymerized, the sample was determined by 1h-nmr. From the chemical shifts and the intensities of resonances of protons, the degree of deacetylation was determined.
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