在摇瓶培养中考察了剪切和供氧对中国红豆杉悬浮细胞生长和紫杉烷积累的影响。
The effects of shear stress and oxygen supply on cell growth and taxane production were investigated in 250ml Erlenmeyer flasks.
试验对影响悬浮细胞生长的因子如培养基中植物生长调节剂组成、初始接种量和继代时间等进行了优化。
Growth of suspension cell lines was affected by the concentration of PGR, the initial cell density and days after sub-cultured, et al.
他的结论表明,对生长缓慢的生物,保持细胞悬浮耗费的能源成本比鞭毛生物少(Walsby 1994年)。
His conclusions show that, for slow-growing organisms, the energy costs for maintaining cells in suspension is substantially less for gas-vesiculate than for flagellated organisms (Walsby 1994).
固体培养和液体悬浮培养的石斛原球茎细胞生长曲线大致呈“S”型。
The growth curve of Dentrobium cell showed "s" type when the protocorm were cultured on solid media or with liquid suspension way.
在此基础上,测定了人参细胞悬浮培养物的生长曲线。
In basis of this, growth curve of ginseng suspension cell was measure.
结果:随着AA剂量的增加,L5178Y细胞的相对存活率和悬浮生长率均明显降低。
RESULTS: The relative survival rate and suspension growth rate of L5178Y cells decreased significantly with the increasing doses of AA.
通过分离、纯化细胞核的对照实验证明不同培养条件对烟草细胞悬浮系的生长和周期具有显著影响。
The controlled experiment of isolating and purifying nuclei proves that the culture conditions work on the growth of the tobacco suspension cells obviously.
结果悬浮培养细胞在M S培养基中细胞生长良好,细胞分散性和喜树碱积累优于在其他培养基中。
Results Suspension culture cells grew well in MS medium and cell homogeneity and camptothecin content were superior to those in other media.
培养条件的优化可有效提高雪莲悬浮细胞的生长和总黄酮的合成。
Cell growth and flavonoids production in the suspention of S. involucrate culture cell should be optimized.
不同愈伤组织的细胞及悬浮细胞在生长过程中细胞形态有差异。
Shape of cell from different callus or suspension cell during different growth phase was diverse.
优化了悬浮细胞系培养的条件:悬浮细胞系的继代时间、谷氨酰胺对悬浮细胞系的生长产生影响。
Culture condition of cell suspension was optimized, the growth of suspension cell was affected by Gin content added and interval of subculture.
首先,摇瓶悬浮培养配子体细胞,考察配子体细胞的生长特性。
Firstly, transgenic kelp gametophyte cells were cultured in the shake flask to find the growth characteristic of gametophytes.
研究了果糖补料和真菌诱导对南方红豆杉细胞悬浮培养体系中细胞生长状态和紫杉烷合成的协同作用。
The results show that synergistic effects of fructose feeding and fungal elicitor changed the cell physiological status, induced alkalinization of medium and decreased the cell biomass.
以处于对数生长初期的悬浮培养细胞为试验材料,研究了在悬浮细胞与毒素亲和性与非亲和性互作早期阶段寄主细胞生理生化的变化。
The physiological and biochemical change of host during initial interaction phase between LA-toxin and suspension cell were studied by using suspension cell within logarithmic growth phase.
结果:倒置显微镜观察,悬浮细胞培养法A组和组织块培养法B组扩增的角膜缘上皮细胞均能在羊膜上单层生长。
Results: A monolayer of cultured corneal limbal epithelial cells was observed by inverted microscope in both A group and B group.
在微载体悬浮培养条件下,脂肪基质干细胞生长更为旺盛,细胞产量更高,优于常规静止培养。
Under the condition of microcarrier suspension culture, the adipose derived stem cells grew more productively, and the production of cell was higher, which were superior to those under static culture.
应用气升式生物反应器及搅拌式生物反应器悬浮培养红豆杉细胞,得到细胞生长和紫杉醇合成动力学曲线。
The growth of plant cell cultures of Taxus chinensis in the airlift bioreactor and stirred tank bioreactor is studied.
U 937是一种前单核细胞,其主要是以悬浮生长为主,细胞形态成圆球状,表面光滑,没有突起。
U937 is a kind of pro monocyte. It grows mainly in the state of suspension.
结论:乳腺癌4t 1细胞可在含多种生长因子的SFM中悬浮生长并形成细胞球,4t1细胞中含有的乳腺癌干细胞样细胞可通过SFM培养法富集。
Conclusion: 4t1 cells can grow and form spheres in serum-free suspension medium containing growth factor, and they contain breast cancer stem-like cells, which can be enriched when cultured in SFM.
这一悬浮细胞系生长快,分散性好,稳定均一,适用于研究其生理、生化和细胞周期调控。
The suspension cell line grew rapidly and was fairly homogenous. It was suitable to be studied on the mechanisms of the plant physiology, biochemistry and the regulation of cell cycle.
目的:通过悬浮适应,使中国仓鼠卵巢细胞(CHO细胞)获得悬浮生长的特性,并可在悬浮培养条件下较快地生长。
Objective: Through suspension adaptation, to endow the Chinese hamster ovary cells (CHO cells) with suspension growth characteristics and rapid growth ability.
细胞悬浮培养一个周期过程中细胞数目增长的动态基本上是一条S型曲线 ,对数生长期明显 ;
The cell number development of Viola diffusa Ging during a culture course was similar to a "S" curve with apparent logarithmic phase.
细胞悬浮培养一个周期过程中细胞数目增长的动态基本上是一条S型曲线 ,对数生长期明显 ;
The cell number development of Viola diffusa Ging during a culture course was similar to a "S" curve with apparent logarithmic phase.
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