否则，即使只有一个等位错配的基因，连接反应也不会发生。
Otherwise even when there is an allele mismatch between them, no ligation would take place.
这不是相信与否，需要在这里没有信仰，这是一个硬连接反应。
It is not something to believe in or not, no faith required here, it is a hard wired response.
叠氮化合物和膦硫酯的无痕施陶丁格连接反应是一种有效生成酰胺键的方法。
The traceless Staudinger ligation of azides and phosphines is an effective method to produce an amide bond.
第一个氨基酸与树脂连接反应是整个合成过程中最关键的反应，它的好坏直接关系到最终产品的产率和纯度。
Attaching amino acid to the resin is the sticking point in the whole synthesis, because it has the most important effects on the yield and purification of ultimate product.
第一个氨基酸与树脂连接反应是整个合成过程中最关键的反应，它的好坏直接关系到最终产品的产率和纯度。
Attaching amino acid to the resin is the sticking point in the whole synthesis, because it has the most important effects on the yield and purification of ultimate product.
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