The physiologic change could take place in the process of forming competent cells.
感受态形成过程中,细胞可能发生了一系列的生理变化。
The products were transformed into BL21 competent cells, coated plates, select monoclonal colony PCR.
连接产物转化至BL21感受态细胞,涂板、挑选单克隆进行菌落PCR。
Objective:Analyzing the affecting factors of forming competent cells of Escherichia coli JM109 to increase transformation efficiency.
目的:分析大肠杆菌JM109感受态形成因素,提高转化效率。
Objective: To establish an efficient and rapid method for the preparation and transformation of competent cells from Escherichia coli.
目的:建立一种高效、快速的大肠杆菌感受态细胞制备及质粒转化的方法。
The immunologically competent cells of main immune organs in chicken vaccinated with HVT and supplemented with Chinese Medicine ImmunologicAdditive in their diets were observed in this study.
研究对鸡马立克氏病火鸡疱疹病毒疫苗(HVT)免疫雏鸡饲喂中药免疫增强剂后,免疫器官中免疫活性细胞的变化进行了观察。
METHODS: Mouse lymphoma EL4 cells were inoculated subcutaneously into wild type C57BL/6 mice (immune-competent mice).
方法:给正常野生型C57BL/6小鼠皮下接种小鼠淋巴瘤EL4细胞,建立荷EL4肿瘤的小鼠模型。
Objective to investigate the transformation efficiency and affecting factors in the preparation of competent E. coli cells by using rubidium chloride (RbCl) method.
目的研究氯化铷法制备感受态细胞的转化效率和不同条件对其的影响。方法采用氯化铷法制备感受态细胞,用不同的质粒进行转化。
Objective to investigate the transformation efficiency and affecting factors in the preparation of competent E. coli cells by using rubidium chloride (RbCl) method.
目的研究氯化铷法制备感受态细胞的转化效率和不同条件对其的影响。方法采用氯化铷法制备感受态细胞,用不同的质粒进行转化。
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