The elution mode, composition of the mobile phase, content of the organic acid and the flow-rate of the mobile phase were optimized and thus a new flow rate gradient was proposed.
对制备色谱的洗脱方式、洗脱剂组成、浓度、洗脱速度等参数进行优化，提出并应用了流动相速度梯度洗脱方法。
The elution mode, composition of the mobile phase, content of the organic acid and the flow-rate of the mobile phase were optimized and thus a new flow rate gradient was proposed.
对制备色谱的洗脱方式、洗脱剂组成、浓度、洗脱速度等参数进行优化，提出并应用了流动相速度梯度洗脱方法。
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