This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
MCHR2; eukaryotic expression vector; transfection; gene expression.
MCHR2；真核表达载体；转染；基因表达。
Conlusion The human FHIT recombinant eukaryotic expression plasmid was constructed successfully.
结论成功构建了重组人FHIT真核表达质粒。
To construct the eukaryotic expression vector of GGT1 and detect its localization in COS7 cells.
构建GGT1重组真核表达载体，观察GGT1在COS7细胞中的定位。
Objective to construct eukaryotic expression plasmid for human insulin-like growth factor 1 (IGF-1).
目的构建人胰岛素样生长因子1 (IGF - 1)的真核细胞表达质粒。
Objective to construct the eukaryotic expression vector of varicella-zoster virus (VZV) glycoprotein e.
目的构建水痘-带状疱疹病毒(VZV)糖蛋白e的真核表达载体。
After mediated by eukaryotic expression plasmid-liposome, the fused protein was introduced into C2C12 cell system.
然后经真核表达质粒-脂质体介导，导入C2C 12细胞系。
Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid.
目的克隆人血管紧张素转换酶2基因（ACE2），并构建其真核表达载体。
Objective to construct an eukaryotic expression vector of compound multi-epitope gene of HCV and express the gene in COS7 cells.
目的建立丙型肝炎病毒(HCV)复合多表位基因的真核表达载体，并在COS7细胞中瞬时表达。
Results rat IL-10 eukaryotic expression vector had been constructed successfully, and had been transfected into rat chondrocyte.
目的构建大鼠il - 10真核表达载体并在大鼠软骨细胞中进行表达。
The result indicated: in this system, the technique of eukaryotic expression vector providing poliovirus capsid protein was possible.
实验结果表明：该系统使用合适的真核表达载体提供脊灰病毒结构蛋白的技术路线是可行的。
Objective To construct the human G250 eukaryotic expression vector and establish the stable B16 cell line expressing human G250 in mice.
目的构建人G250真核表达载体，建立稳定表达人G250的小鼠黑色素瘤细胞系。
Objective to construct eukaryotic expression vector of antisense MBD1 gene fragment and to provide a tool for studying MBD1 gene function.
目的构建反义MBD1基因片段真核表达载体，为研究MBD1基因功能提供工具。
Objective: to construct eukaryotic expression vector of human death receptor (DR5) and transfect NS-1 cells to establish stable NS-1 cell line.
目的：构建人死亡受体5 (DR5)真核表达载体，转染NS - 1细胞，建立稳定转染的NS - 1细胞系。
Introduction The construction and transfection of eukaryotic expression plasmid are commonly molecular biology method used in medical research.
序言真核表达质粒的构建和转染是目前医学研究中常用的分子生物学手段。
Aim to clone human CD81 gene from peripheral blood lymphocytes, and construct its eukaryotic expression vector, and then express it in COS-7 cells.
目的从人外周血淋巴细胞中克隆出cd 81基因，构建真核表达质粒，并在COS - 7细胞中进行表达。
The adenoviruses and the eukaryotic expression plasmid have unique practicality in preparing tumor treating medicines and radiotherapy hypersensitivity.
该腺病毒和真核表达质粒在肿瘤治疗药物、放射治疗增敏的制备中具有独特的实用价值。
Objective: to establish NK cell receptor NKG2D eukaryotic expression vector and investigate on the cytotoxicity of NK cell line-YT transfected with NKG2D.
目的建立NK细胞受体nkg2d真核表达载体，通过转染NK细胞系yt，初步探讨NKG2D分子对YT细胞系杀伤功能的增强作用。
Objective to construct eukaryotic expression vector of human pancreatic duodenal homeobox 1 (PDX-1) gene, and to detect its expression in NIH3T3 cell lines.
目的构建人胰十二指肠同源盒(PD X - 1)基因的真核表达载体，观察其在NIH3T3细胞中的表达。
AIM: to construct eukaryotic expression vector carrying human antisense VEGF gene and to study its effect on VEGF expression and growth of renal cell carcinoma.
目的：构建反义vegf基因真核表达载体，研究其对肾癌细胞VEGF表达的影响。
Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.
结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
To clone mouse interleukin 4 (mIL-4) truncated gene, construct its eukaryotic expression plasmid pFB-mIL4 and express the truncated protein (murine IL-4 receptor antagonist).
构建小鼠il -4截短型基因真核表达质粒，表达小鼠il - 4受体拮抗体蛋白。
This research intended to construct eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene, and verify its expression efficacy in C2C12 cells.
本研究旨在克隆通城猪含有第1个内含子的MSTN前肽基因，构建真核定点诱变载体，并通过转染C2C12细胞验证载体表达的有效性。
Conclusion Construction of the eukaryotic expression vectors of wild type and mutant PRPF31 genes is basic work for research on the mechanisms of retinitis pigmentosa caused by PRPF31 mutation.
结论野生型和突变型PRPF31基因真核表达载体的构建，为研究PRPF31基因突变引起视网膜色素变性的机制奠定了基础。
That cell hybridization can be used to dissect regulatory mechanisms controlling gene expression in eukaryotic cells.
杂交细胞能被应用于剖析真核细胞中控制基因表现的调节机理。
What are the most significant differences between the organization and expression of prokaryotic genes and eukaryotic genes?
原核生物的基因与真核生物的基因在组织形式和表达方式方面有哪些主要的区别？
Eukaryotic genome is packaged into chromatin in the nucleus. There must be some change at chromatin level during gene expression regulation.
真核细胞基因组以染色质状态存在于细胞核内，基因的表达调控首先要在染色质水平发生变化。
As the key point of expression modulation in prokaryotic and eukaryotic cells, the protein phosphorylation and dephosphorylation may help reveal the status of the life system at the molecular level.
蛋白质磷酸化和去磷酸化作为原核和真核细胞表达调控的关键环节，了解其对功能的影响可以深入理解生命系统在分子水平的调控状况。
Recently, with the development of transgenic technology, producing transgenic animal model has become an important way to study the expression regulation of eukaryotic genes.
近年来，随着转基因动物制备技术的日趋成熟与完善，建立转基因动物模型已经成为研究真核基因表达调控的一种重要手段。
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