The paper describes process of recombinant fusion protein in purification and existing problems.
本文论述了基因重组融合蛋白纯化过程和存在的问题。
The results showed that the fusion protein can yield antibodies with high neutralizing activity as well as provide protection against challenge with virulent virus.
结果表明，用此融合蛋白的包涵体免疫豚鼠能诱导产生中和抗体，并对病毒的攻击提供免疫保护。
Monoclonal antibody is produced by immunizing animals with a fusion protein containing the cytoplasmic domain of human EGF receptor.
该单克隆抗体是通过用包含人EGF受体胞质域的融合蛋白免疫动物而制备的。
The inhibition ratio of the fusion protein was tested by MTT method.
MTT法检测融合蛋白对黑色素瘤细胞的体外抑制率。
Conclusion The GRA7 gene may be expressed as a GST fusion protein in E. coli.
结论GRA7基因在大肠埃希菌中以gst融合蛋白的形式得到表达。
Cell density cultivation and high level expression of recombinant urease a fusion protein in Helicobacter pylori.
目的高密度培养重组细菌和高效表达重组幽门螺杆菌尿素酶a融合蛋白。
Then, a fusion protein expression system was used to express the TNF half molecule.
用融合蛋白表达系统对该半分子进行大肠杆菌表达。
Monoclonal antibody is produced by immunizing animals with a GST fusion protein.
单克隆抗体通过合成GST融合蛋白免疫动物制备。
Neuropeptide y; Fusion protein; Inclusion body; Purification; Bioinformatics.
人神经肽y；融合蛋白；包涵体；纯化；生物信息学。
Objective to construct an apoptin expression system to produce an antigen, apoptin fusion protein.
目的构建凋亡素原核表达系统，以制备抗原物质凋亡素融合蛋白。
Results: The CAT gene was cloned correctly and it's fusion protein was expressed in E.
结果：成功克隆了CAT的基因片段，并在大肠杆菌中得到其融合蛋白的表达。
This fusion protein prevents certain blood cells from maturing and leads to an accumulation of immature leukemia cells.
这一融合蛋白阻止特定血液细胞分化成熟，使幼稚细胞堆积。
If the fusion protein of VP1 and VP2 that was expressed in E. coli can form neutralizing antigen epitopes, this problem is resolved.
如果大肠杆菌中表达的VP1和VP2融合蛋白也能形成中和抗原表位，则解决了这个问题。
CONCLUSION: The mouse endostatin recombinant fusion protein expressed in E. coli by using thioredoxin fusion expression system is easy to be purified and possesses high activity.
结论：用硫氧还蛋白融合表达系统在大肠杆菌中表达的小鼠内皮抑素重组融合蛋白易纯化并具有高活性。
RESULTS: Fusion protein existed in supernatant of the bacteria lysate and its expression level was about 7% of the total bacteria protein.
结果：融合蛋白以可溶性形式存在于细菌裂解液的上清中，其表达量为菌体总蛋白量的7%。
Monoclonal antibody is produced by immunizing animals with a BMP4 fusion protein.
单克隆抗体由BMP4融合蛋白免疫动物产生。
The PP65 protein was expressed on the surface of the T7 as head fusion protein. There were 5 to 15 copies on the each phage.
PP 65目的蛋白以融合蛋白的形式表达在T7噬菌体的头蛋白部位，每个噬菌体颗粒表面可表达5 ~15个拷贝。
Membrane fusion within the Paramyxoviridae family of viruses is mediated by a surface glycoprotein termed the "f", or fusion protein.
副粘病毒科病毒的膜融合是由其表面糖蛋白，F蛋白介导的。
Purpose to study the effect of different factors on the efficiency of cleaving fusion protein with cyanogen bromide.
目的研究不同因素对溴化氰切割融合蛋白的影响。
The fusion protein was obtained by purifying protein a.
蛋白a纯化获得电泳纯融合蛋白。
The hematopoietic and chemotactic activities of the fusion protein were investigated in vitro.
研究了该融合蛋白在体外的促造血细胞增殖活性和对免疫细胞的趋化活性。
A new medicine in development for the wet form of AMD is a recombinant fusion protein that binds to the growth factor protein that plays a critical role in blood-vessel formation in the eye.
一种在研治疗湿性amd的新药是一种重组融合蛋白，它能结合眼内血管形成关键生长因子蛋白。
Fusion protein expression was induced by IPTG. After renaturation, the protein was purified by affinity chromatography and the bioactivity was examined by ELISA.
IPTG诱导表达融合蛋白，包涵体蛋白经复性后亲和层析法纯化，ELISA方法测定蛋白活性。
Results the fusion protein expressed in e. coli account for 20% of the total bacterial protein.
结果融合蛋白的表达量占菌体总蛋白的20%。
Objective: To express and purify the fusion protein of galectin-1.
目的：表达和纯化半乳糖凝集素- 1融合蛋白。
Fusion protein is the main protective antigen of NDV and the primary structure of fusion protein determines the virulence of the virus.
蛋白不仅是NDV主要保护性抗原，而且F蛋白一级结构(氨基酸顺序)决定病毒毒力。
The purity of fusion protein was about 70% after ultrasonic decomposition and washing repeatedly, and reached above 95 % after purification by ion exchange and gel-filtration chromatography.
菌体经过超声破碎后反复洗涤包涵体，融合蛋白纯度达到70%，进一步用离子交换层析和凝胶过滤层析纯化使其纯度达95%以上。
RESULTS: DDR2/EGFP fusion plasmid was successfully constructed. Further analysis also demonstrated that the fusion protein has similar expression and activation pattern with wild type ones.
结果：成功构建了DDR2/EGFP融合表达载体，进一步的分析也证明此融合表达载体能在细胞中正确表达并可以被配体所激活。
RESULTS: DDR2/EGFP fusion plasmid was successfully constructed. Further analysis also demonstrated that the fusion protein has similar expression and activation pattern with wild type ones.
结果：成功构建了DDR2/EGFP融合表达载体，进一步的分析也证明此融合表达载体能在细胞中正确表达并可以被配体所激活。
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