Vitamin D receptor (VDR) gene polymorphism was detected using PCR-RFLP.
RFLP法检测维生素D受体（VDR）基因多态性。
Conclusion PCR-RFLP is an accurate, specific method for HSV diagnosis and typing.
结论P CR - RFLP法是一种准确、特异的对单纯疱疹病毒感染进行诊断和分型的方法。
The polymorphisms of MGP gene and ALAD gene were analyzed by the methods of PCR-RFLP.
采用多聚酶链反应-限制性片段长度多态性法(PCR - RFLP)分析MGP和ALAD基因的多态性。
Subsequently, 11 samples were selected for restriction fragments length analysis (PCR-RFLP).
随后，对11份标本的PCR-RFLP结果进行了分析。
Methods: PCR-RFLP technique was used for detecting the A677V polymorphism site of MTHFR gene.
方法：运用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR - RFLP)检测MTHFR的677位点多态性。
Interestingly, both mutant alleles had a single-base deletion, which was confirmed by PCR-RFLP.
有趣的是两个突变体中都发生了单个碱基的缺失，PCR-RFLP证实了该结果。
As the inheritance variation is universal in parasites, the precise research with PCR-RFLP in parasites is of importance.
寄生虫的遗传变异现象十分普遍，用P CR - RFLP技术精确分析寄生虫遗传变异的研究意义重大。
Conclusion PCR-RFLP is a promising molecular biological technique, which could rapidly and correctly classify Malassezia species.
结论P CR - RFLP法是一种有希望能够快速、准确对马拉色菌属进行种间分类的分子生物学技术。
ResultsThe results of genotyping in two-step PCR-CTPP were consistent with those conducted by PCR-RFLP and DNA sequence analysis.
结果通过两步法PCR-CTPP得到的基因分型结果与PCR-RFLP和DNA测序得到的基因分型结果完全一致。
Methods CYP1A1 and GSTM1 genetic polymorphisms were determined by PCR-RFLP in 163 lung cancer cases and healthy controls respectively.
方法用PCR-RFLP技术分析了原发性肺癌组和住院对照组（各163例）的CYP1A1、GSTM1基因的多态性、基因型分布频率和交互作用。
Conclusion a higher level of consistency and repeatability was found from the method of semi-quantitative PCR-RFLP compared to PCR-RFLP.
结论应用半定量pcr方法可明显提高P CR - RFLP酶切法的精确性和可重复性。
Methods:The method for MBL point mutation detection(PCR-RFLP) was Established with self-designed primers according to MBL genomic sequence;
方法：根据MBL基因序列设计引物建立MBL基因点突变检测方法即PCR -RFLP ；
To identify poliovirus isolated from feces of acute flaccid paralysis case type in gene. Isolated strains were identified by PCR-RFLP method.
对急性驰缓性麻痹病例粪便标本分离的脊髓灰质炎病毒进行型内鉴定。
Methods:Mutations of multiple genes from 106 patients with SCC were analyzed by two multiplex PCR-SSCP systems, PCR-RFLP, and DNA sequencing.
方法：对10 6例散发性大肠癌患者进行多基因突变和肠道内环境中有关指标（以粪便为标本）的测定，并进行流行病学的病例-病例研究分析。
Paraoxonase 2 (PON2) gene polymorphism (C311S at exon 9) was determined by PCR-RFLP in 327 type 2 diabetic patients with various degrees of albuminuria.
采用PCR RFLP法检测了327例不同程度白蛋白尿的2型糖尿病患者对氧磷酶2（PON2）基因第9 外显子的C311S多态性。
Methods ER genotyping was performed in 448 healthy people by PCR-RFLP and compared the distribution of genotype in Chongqing with different areas and races.
结论ER基因型的分布在不同地域、不同种族间存在显著差异，这种差异可能与某些疾病在不同地域、不同种族间不同的发病率和临床表现有关。
This paper summarizes the application of molecular biological techniques in the identification of Taenia, such as analysis of DNA sequence, PCR-RFLP and LAMP.
本文综述了DNA序列分析、P CR限制性片段长度多态性技术和环介导等温扩增技术在带绦虫虫种鉴别中的应用。
PCR-RFLP was used to analyze 360 chromosomes from 180 Chinese unrelated healthy Han individuals, and the analysis of the genotypes of members in six families.
采用PCR-RFLP方法，对180例无血缘关系的健康中国汉族个体的360条染色体和6个家系18位成员的36条染色体进行检测。
Methods:336 hypertension patients were recruited in this trail. The distribution of ACE2 gene A9570G polymorphism was analyzed by PCR-RFLP in all participants.
方法：选择符合入选标准的高血压患者336例，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的方法，进行ACE2基因分型。
Methods Using multiple PCR and PCR-RFLP methods, we studied the NAT1 genotypes and its genetic polymorphisms of the peripheral blood samples from 140 Han people.
方法在140名汉族健康人的外周血中，应用聚合酶链反应（PCR）限制性片段长度多态性分析（RFLP）及多重PCR技术，进行NAT1等位基因分型研究。
Pvu II and Xba I restriction fragment length polymorphisms (RFLP) of ER were analysed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) .
分析雌激素受体基因多态性与骨密度的关系及各基因型在骨质疏松组与对照组的分布。
It also manifested that PCR-TGGE is superior to PCR-RFLP for detail dynamics of soil microbial community, and it is a rapid method to discriminate multiple samples simultaneously.
比较两种分子生物学方法的结果表明，PCR TGGE技术比pcr RFLP技术更能精确地反映土壤微生物的动态变化，并且能同时快速地对多个样品进行有效区分。
Methods PCR-RFLP were used to examine the single nucleotide polymorphism (SNP) of GPRA gene in118children with bronchial asthma (case group) and124healthy individuals (control group).
方法采用PCR - RFLP方法检测，并分析118例儿童支气管哮喘患者和124例健康对照儿童gpra基因多态性。
With BMPR-IB gene as candidate gene, its polymorphism distribution in Suffolk sheep, small-tail Han sheep and their F1 hybrid germination were detected by using the method of PCR-RFLP.
以BMPR - IB基因作为候选基因，采用PCR - RFLP方法检测其在萨福克羊、小尾寒羊及萨寒f 1代杂交羊中的多态性分布，分析其与产羔数的相关性。
Methods We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) to examine 40 oral squamous cell carcinomas for loss of heterozygosity (LOH) at APC.
方法应用聚合酶链反应限制性片段长度多态性分析方法(PCR - RFLP)对40例口腔鳞癌组织中apc基因的杂合缺失(LOH)进行检测。
Conclusions: PCR-RFLP is a sensitive, specific and rapid method in identification of mycobacterium. Application of this method will be helpful for early diagnosis of mycobacterial skin infections.
结论：用PCR-RFLP可以快速鉴定海分枝杆菌，有利于该类疾病的早期诊断和及时治疗。
Methods One family and 120 sporadic patients with Parkinson's disease were studied using polymerase chain reaction, DNA sequencing and restriction fragment length polymorphic (PCR-RFLP) techniques.
方法应用聚合酶链反应（ PCR）、DNA测序和限制性片段长度多态性（ RFLP）等技术对1个帕金森病家系及120例散发性帕金森病患者进行PINK1基因R492X的突变分析。
The genetic variation of E. coli F18 receptor (ECF18R) loci in 867 pigs including Large Yorkshire, Landrace, Duroc, Ningxiang pig, Shaziling pig and Daweizi pig was detected by PCR-RFLP with Hin6 I.
采用PCR-RFLP技术检测了大约克、长白、杜洛克、宁乡、沙子岭和大围子6个品种共867头猪的E．coli F18受体（ECF18R）基因座的遗传变异。
FISH and RFLP are the most Labour intensive, time consuming and more expensive methods. The special PCR markers, in some degree, are effected by the content of template DNA.
FISH和RFLP方法比较费时费力且花费昂贵，而特异性P CR标记又在一定程度上受模板DNA浓度的影响。
FISH and RFLP are the most Labour intensive, time consuming and more expensive methods. The special PCR markers, in some degree, are effected by the content of template DNA.
FISH和RFLP方法比较费时费力且花费昂贵，而特异性P CR标记又在一定程度上受模板DNA浓度的影响。
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