Methods: 85 clinical strains of S. aureus were extracted by phenol chloroform method and the RAPD typing was also performed.
方法:对临床分离的85株金黄色葡萄球菌,用酚氯仿法提取DNA后进行RAPD分型。
The chloroform solution of 4-Amino-antipyrine was used to measure the phenol in water by extraction and photometer. The method and the precision were discussed in this paper.
本文通过实验的方法,用三氯甲烷处理4- 氨基安替比林溶液,解决了用4-氨基安替比林测定挥发酚实验空白值高的问题,并确定了三氯甲烷最佳使用量。
Objective To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping.
目的建立运用酚-氯仿法结合磁珠法从蝇蛆嗉囊内提取人类DNA的方法,从而提高STR分型检验的灵敏度。
Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA,比较荧光定量pcr测定的重复性和测定值的差异。
METHODS DNA from 15 clinical strains of S. aureus were extracted by phenol-chloroform method and used in the RAPD assay, phage typing were also performed.
方法对临床分离的15株金黄色葡萄球菌,酚氯仿法提取其DNA后行RAPD分型,同时进行噬菌体分型。
The results suggest that this method, though simple, can be used to prepar template for PCR from various cells and tissues without extracting DNA by SDS-phenol-chloroform.
揭示该方法能从各种细胞和组织中制备pcr的模板,而无需用SDS -苯酚-氯仿抽提dna。
DNA was extracted from stools by phenol chloroform extracting method.
通过改良酚氯仿抽提法提取粪便dna。
The result shows that routine phenol-chloroform method was adapt to extract a mass of DNA.
结果表明,常规酚—氯仿抽提法适合从马血中大量提取DNA。
The result shows that routine phenol-chloroform method was adapt to extract a mass of DNA.
结果表明,常规酚—氯仿抽提法适合从马血中大量提取DNA。
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