Methods: HBV genotype was tested in 163 patients by real-time PCR.
方法 采用荧光PCR法对163例乙肝患者血清进行基因分型。
Real-time PCR reaction; Amplification curve output; Ct data obtain;
进行实时定量PCR反应，建立标准曲线；
Moreover, to detect the mitochondria DNA copy Numbers of sample with real-time PCR.
用实时定量pcr检测标本线粒体dna的拷贝数。
To explore the method of preparation for Hantavirus s segment standard plasmids of real-time PCR.
构建汉坦病毒S片段标准品，用于实时荧光定量pcr检测汉坦病毒。
Conclusions Real-time PCR is a reliable tool for the validation of high through-put data such as SAGE.
结论实时定量pcr技术是验证sage等高通量数据的可靠工具之一。
Two real-time PCR assays for specific detection of Mycobacterium tuberculosis and Mycobacterium bovis were developed.
目的对结核分枝杆菌和牛分枝杆菌分别建立荧光PCR快速检测方法。
Myocardial ACE2 mRNA expression was respectively determined by real-time PCR (RT-PCR) in control group and diabetes group.
采用实时定量pcr (RT - PCR)检测正常及糖尿病大鼠心肌组织ace2的基因表达。
Methods Choose correct and effective PCR cycle number according to standard plasmid's amplification curve in real-time PCR;
方法根据标准质粒荧光定量PCR的扩增曲线选择合适有效的扩增循环次数；
Multiplex PCR and real-time PCR are developed for simultaneous and quantitative detection of bacteria-producing biogenic amine.
利用多重PCR和实时定量PCR技术可以实现对葡萄酒中生物胺产生菌的快速定量检测。
Objective To quantitatively analyse the relationship between uncultivated phylotype AU126 and periodontal disease with real-time PCR.
目的运用实时P CR技术，对未获培养微生物au126与牙周病的关系进行定量分析。
We investigated the presence of these atypical pathogens in sputum samples in patients with stable COPD and those with AECOPD using real-time PCR.
据最近一项来自欧洲呼吸杂志研究表明：非典型呼吸道病原体在慢性阻塞性肺疾病恶化加剧中并无意义。
The products of ruminant feed and cattle or sheep-derived feed from the enterprises and farms of 7 districts in Shaanxi province were detected by real-time PCR.
实时PCR对陕西省7个地市生产、经营和使用的反刍动物饲料产品和动物源性饲料产品（原料）进行了牛羊源成分检测调查。
The molecule biological techniques DGGE, clone and real-time PCR were utilized to study prinimilarily the microorganism in 2 anaerobic ammonia oxidation reactors.
利用变性梯度凝胶电泳、克隆和实时PCR等分子生物学技术对2个厌氧氨氧化反应器中的微生物进行了初步研究。
Objective To set up a duplex quantitative real-time PCR (QPCR) assay with high sensitivity, specificity and rapidity to detect Candida krusei and Candida glabrata.
目的建立一种快速、灵敏、特异的鉴定克柔念珠菌和光滑念珠菌的双重实时荧光定量PCR方法。
Methods Serum samples from 188 chronic hepatitis B patients with lamivudine therapy were collected and quantitatively tested with real-time PCR for HBV DNA and YMDD mutations.
方法将接受拉米夫定治疗的188例患者根据治疗时间进行分组，采用实时荧光PCR方法定量检测各组患者血清HBVDNA水平和酪氨酸-甲硫氨酸-天冬氨酸-天冬氨酸（YMDD）变异。
The aim of this assay is to investigate on the TaqMan Real-time PCR method for phytoplasma general detection which has the superiorities in saving time and preventing contamination.
本实验意在研究一种能够应用于植原体初筛，并且不易污染，易操作耗时短的通用方法。
This real-time PCR reaction system is highly specific, reliable, high sensitivity for detecting pathogenic fungus which could be useful for the diagnosis of clinical fungus infection.
建立的实时荧光PCR方法能特异、灵敏的检测临床病原真菌，为临床真菌感染的诊断提供了一种快速、准确的方法。
Here we present the results of the study on the detection of Influenza virus in the ambient air contaminated with high concentrations of bacteria and fungi using real-time PCR protocol.
本篇中成功于一高浓度细菌及真菌之大气环境中建立侦测流感病毒之技术。
The results of real-time PCR analysis revealed that TAFFC transcripts were steady in incompatible interaction of wheat and stripe rust, whereas down-regulated in compatible interaction.
实时荧光定量PCR结果表明，该基因在受到条锈菌诱导下，在亲和组合中表达趋势明显下调，而在非亲合组合中的表达趋势比较稳定。
Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.
方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。
The autopsy samples, including heart, liver, spleen, lung, kidney and brain, were ground and detected by Real-time PCR. According to the standard curve, virus load of H5N1 AIV was calculated.
将人禽流感死亡病例的尸解标本，包括心、肝、脾、肺、肾、脑等组织标本研磨处理后，进行荧光定量pcr检测，并根据标准曲线推算H5N1禽流感病毒的病毒载量。
Finally, the expression levels of the candidate genes were further confirmed by quantitative real-time PCR using a new set of samples (20 narcolepsy-cataplexy patients and 20 healthy controls).
最后，用定量实时PCR法在一组新样本(20名发作性睡病-猝倒患者和20名健康对照)中进一步确认候选基因的表达水平。
Each lab now has a real-time polymerase chain reaction (PCR) machine, and one lab has two machines.
每个实验室都有一台实时多聚酶链式反应机，有一个实验室有两台这样的机器。
All had been evaluated using a slower but highly accurate test called real-time reverse transcription-polymerase chain reaction, or rRT-PCR, which checks for the genetic material of the virus.
应用一种缓慢但高度准确的试验，检测病毒的基因物质，叫即时逆转录-聚合酶链反应，或是rRT - P CR，全部做了评价。
Results The sensitivity of the established real time quantitative PCR was at 10 -4 level.
结果建立的实时荧光定量PCR方法的灵敏度为10-4水平。
Results The sensitivity of the established real time quantitative PCR was at 10 -4 level.
结果建立的实时荧光定量PCR方法的灵敏度为10-4水平。
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