To sequence the polymorphic gene found by RFLP technique.
对筛查出的多态性基因进行测序分析。
In avian RFLP analyses, mtDNA are widely used as target sequences.
在鸟类的RFLP分析中,用的最多的靶序列是线粒体dna。
HBV genotypes were determined by RFLP based on S-gene PCR products.
用S基因聚合酶链反应-限制性片段长度多态性确定HBV基因型。
Conclusion PCR-RFLP is an accurate, specific method for HSV diagnosis and typing.
结论P CR - RFLP法是一种准确、特异的对单纯疱疹病毒感染进行诊断和分型的方法。
Subsequently, 11 samples were selected for restriction fragments length analysis (PCR-RFLP).
随后,对11份标本的PCR-RFLP结果进行了分析。
Interestingly, both mutant alleles had a single-base deletion, which was confirmed by PCR-RFLP.
有趣的是两个突变体中都发生了单个碱基的缺失,PCR-RFLP证实了该结果。
But the RFLP markers on the map are not evenly distributed and there are two breaking points on chromosomes 4 and 8.
但该图的RFLP标记分布不够均匀,存在较多的空白区,特别是在第4和第8号染色体上存在两个断点。
As the inheritance variation is universal in parasites, the precise research with PCR-RFLP in parasites is of importance.
寄生虫的遗传变异现象十分普遍,用P CR - RFLP技术精确分析寄生虫遗传变异的研究意义重大。
RFLP analysis was carried out on 36 rice elite line. Sterility of these 162 rice hybrids was studied in groups and in types.
对36个水稻骨干系进行了RFLP(限制性内切酶长度多态性)分析,并对这些水稻材料的162个杂交组合育性进行了分组分类研究。
Conclusion PCR-RFLP is a promising molecular biological technique, which could rapidly and correctly classify Malassezia species.
结论P CR - RFLP法是一种有希望能够快速、准确对马拉色菌属进行种间分类的分子生物学技术。
ResultsThe results of genotyping in two-step PCR-CTPP were consistent with those conducted by PCR-RFLP and DNA sequence analysis.
结果通过两步法PCR-CTPP得到的基因分型结果与PCR-RFLP和DNA测序得到的基因分型结果完全一致。
RFLP is one of the main molecular marking means, and it plays a very important role in researches of plant heredity and breeding.
RFLP作为一种主要的分子标记手段,在植物遗传育种研究中占有重要的地位。
Methods CYP1A1 and GSTM1 genetic polymorphisms were determined by PCR-RFLP in 163 lung cancer cases and healthy controls respectively.
方法用PCR-RFLP技术分析了原发性肺癌组和住院对照组(各163例)的CYP1A1、GSTM1基因的多态性、基因型分布频率和交互作用。
Conclusion a higher level of consistency and repeatability was found from the method of semi-quantitative PCR-RFLP compared to PCR-RFLP.
结论应用半定量pcr方法可明显提高P CR -RFLP酶切法的精确性和可重复性。
Compared the selective efficiency of RAPD, RFLP and AFLP markers in genetic background selection, AFLP marker was found to be the ideal one.
对实施背景选择的RAPD、RFLP和AFLP标记进行了比较,认为AFLP标记是一种理想的辅助背景选择的分子标记。
A set of RFLP landmarks generated by the Japanese Rice Genome Program (RGP) was applied to allow comparison between our map and the RGP map.
由于采用了一套日本水稻基因组计划(rgp)的RFLP标记,使本图谱能与RGP图谱进行比较。
To identify poliovirus isolated from feces of acute flaccid paralysis case type in gene. Isolated strains were identified by PCR-RFLP method.
对急性驰缓性麻痹病例粪便标本分离的脊髓灰质炎病毒进行型内鉴定。
The purpose of this paper is to introduce the fundamentals of RFLP, RAPD techniques and their applications in plant breeding and genetic studies.
本文对RFLP和RAPD等分子标记技术原理及其在植物遗传育种中的应用作一综述。
The application of T - RFLP microbial monitor technique in the field experiment of the microbial flooding was introduced in Luo 801 block, Luojia Oilfield.
文章介绍了T-RFLP微生物监测技术在罗家油田罗801块微生物驱油现场试验中的应用情况。
Its applications were limited for the low polymorphism in the gene group of barley using the labeling technique of morphology, cytology, isoenzyme and RFLP.
由于形态学、细胞学、同工酶、RFLP等标记技术在大麦基因组中的多态性较低,限制了其应用。
This paper summarizes the application of molecular biological techniques in the identification of Taenia, such as analysis of DNA sequence, PCR-RFLP and LAMP.
本文综述了DNA序列分析、P CR限制性片段长度多态性技术和环介导等温扩增技术在带绦虫虫种鉴别中的应用。
PCR-RFLP was used to analyze 360 chromosomes from 180 Chinese unrelated healthy Han individuals, and the analysis of the genotypes of members in six families.
采用PCR-RFLP方法,对180例无血缘关系的健康中国汉族个体的360条染色体和6个家系18位成员的36条染色体进行检测。
In recent years, the application of molecular markers such as RAPD, AFLP, and RFLP markers have speeded up the step of olive genetic resource and breeding study.
近年来,RAPD、AFLP和RFLP等分子标记技术的应用,加速了油橄榄种质资源和育种研究的步伐。
Methods Using multiple PCR and PCR-RFLP methods, we studied the NAT1 genotypes and its genetic polymorphisms of the peripheral blood samples from 140 Han people.
方法在140名汉族健康人的外周血中,应用聚合酶链反应(PCR)限制性片段长度多态性分析(RFLP)及多重PCR技术,进行NAT1等位基因分型研究。
With the strong- points of abundant polymorphism, repeatable operation and carrying creaible inheritance information, PCR- RFLP has been researched and applied actively.
由于其具有丰富的多态性、操作的可重复性、遗传信息的忠实性等优点,PCR-RFLP技术的研究和应用十分活跃。
FISH and RFLP are the most Labour intensive, time consuming and more expensive methods. The special PCR markers, in some degree, are effected by the content of template DNA.
FISH和RFLP方法比较费时费力且花费昂贵,而特异性P CR标记又在一定程度上受模板DNA浓度的影响。
RFLP patterns were analyzed from 59 cultivars of Brassica napus, including 46 Chinese and 9 European accessions, using 28 genomic probes of Brassica and 2 restriction enzymes.
以甘蓝型油菜的28个基因组探针和两种限制性内切酶对包括46个中国品种、9个欧洲品种在内的59个甘蓝型油菜品种(系)的RFLP标记进行了分析。
Methods Single round Touchdown PCR (TD PCR) and restriction fragment length polymorphism (RFLP) method were used for genotyping in 110 people from the Han population in Chongqing.
方法对110名重庆汉族个体,采用降落式PCR和限制性片段长度多态性法(RFLP)进行基因型分析。
Methods Single round Touchdown PCR (TD PCR) and restriction fragment length polymorphism (RFLP) method were used for genotyping in 110 people from the Han population in Chongqing.
方法对110名重庆汉族个体,采用降落式PCR和限制性片段长度多态性法(RFLP)进行基因型分析。
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