PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
After subculture, confluence was occurred in 1-2 weeks.
传代培养细胞可在1 ~2周汇合成片。
Anime subculture has a distinctive effect on juvenile delinquency.
青少年亚文化对青少年犯罪的影响极为显着。
Websites devoted to the phones are appearing and have become a genuine subculture.
为山寨机设立的网站不断出现，成为一种真正的亚文化现象。
However, to brand culture, subculture is an important carrier of communication.
而对于品牌文化来说，亚文化是一个重要的传播载体。
Once simply a bizarre subculture in Japan, cosplay is becoming a global phenomenon.
动漫真人秀曾经还只是一种标新立异的日本亚文化，而今正在成为一种全球现象。
The unhealthy subculture of college students has its basic form and characteristics.
大学生不良亚文化有其基本的表现形式和特点。
Freedom isn't about a subculture; it's about the culture. It's not a microcosm; it's a cosm.
《自由》写的不是亚文化而是关于文化；不是微小世界而是大世界。
Somatic clones were established after 15 generations of subculture for more than two years.
经过两年多(15个世代)的继代培养，建立了党参体细胞无性系。
As a kind of subculture, Enterprise culture is also influenced by Chinese Traditional culture.
企业文化作为一种亚文化，受到传统文化的深远影响。
Rather, they believe they are a subculture which has its own language, American Sign Language.
他们颇有点儿认为他们是一个亚文化，有自己的语言——美国手语。
The film concerns itself with the subculture of the film buff. Everything rotates around film.
影片关注的是电影爱好者亚文化群，一切都围绕着电影进行。
Many embryogenic calli were produced by subculture of calli induced from mature embryo of rice.
从水稻成熟胚诱导出的愈伤组织经继代培养后可以产生大量的胚性愈伤组织。
The subculture is playing an ever important role in the process of communication of brand culture.
亚文化群体在品牌文化的传播过程中，扮演着越来越重要的角色。
Star Wars has leaked into nearly every subculture since its premiere in 1977, and steampunk is no exception.
自1977年首映以来，《星球大战》几乎渗入到每一种亚文化之中，蒸汽朋克也不例外。
The spiritual subculture of the West has for the longest time advocated a balance of spirituality and material life.
灵性的西方分支主张灵性与物质生活的平衡是最长期的了。
"It gave me some insight into that mindset, that subculture, but I never want to see it again," he said. "Once was enough."
这部电影让我对那种心态，那种亚文化有了一些了解，但我再也不想看了，一次足矣。
Information culture, chase-star culture, communication patterns of the peer groups are the forms of campus subculture.
信息文化、追星文化、同辈群体交往模式是校园亚文化最突出的表现形式。
"The Gulag Archipelago" described the system, its tortures, rules and subculture, in relentless, gruesome, encyclopedic form.
《古拉格群岛》描述的系统，是充满折磨，统治和亚文化的，以一种无情的，可怕的，包罗万象的形式存在的系统。
Campus culture, a subculture of the social main culture, functions as the shaper of people's spirit, soul and personality.
校园文化是社会主体文化的亚文化，它的职能是对人的精神，心灵、性格的塑造。
Hollow Ones: a group that espouses post-modernism, the Gothic subculture and decadence in response to the declining world.
虚无党：法师团体，支持用后现代主义、哥特文化和颓废行为来回应这个日益败落的世界。
Nisan is part of a thriving subculture of men and women in Japan who indulge in real relationships with imaginary characters.
尼森现象只是一种在日本男女中间兴起的亚文化，他们沉迷于和虚拟人物的关系之中。
Also on view are pieces showcasing goth's different subculture fashions, like Gothic Lolita, cyber-goth, old-school goth, and Victorian-goth.
一同展出的还有反映哥特风格中各种亚文化风尚的作品，如哥特式洛丽塔，超现实哥特，老派哥特和维多利亚·哥特。
Also on view are pieces showcasing goth's different subculture fashions, like Gothic Lolita, cyber-goth, old-school goth, and Victorian-goth.
一同展出的还有反映哥特风格中各种亚文化风尚的作品，如哥特式洛丽塔，超现实哥特，老派哥特和维多利亚·哥特。
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