The second issue is that the gene vector, in this case the plasmid, has to be compatible with the cells that you're trying to express the gene in.

接下来的问题是如何选择合适的基因载体,在这里用到的,质粒,必须与要用来表达基因的,受体细胞匹配

耶鲁公开课 - 生物医学工程探索课程节选

But if you're trying to use that virus as a gene delivery vector and your body gets rid of it then the therapy has failed, it's ended.

但如果你想把腺病毒作为基因治疗载体,而你的身体清除这种病毒,治疗就失败了,前功尽弃了

耶鲁公开课 - 生物医学工程探索课程节选

That's another advantage of using viruses as a gene therapy vector.

这是病毒,作为基因疗法载体的另一个优势

耶鲁公开课 - 生物医学工程探索课程节选

Those two examples I just gave you, one had to directly inject a gene containing vector into the pronucleus of a fertilized egg and you don't have that opportunity in adults.

在我刚给出的两个例子里,一个必须得将带有目的基因的载体,直接注射到受精卵的原核中,这在成年人身上行不通

耶鲁公开课 - 生物医学工程探索课程节选

One of the ways that it's used is to take the fertilized egg from an animal, a mouse, usually it's a mouse and inject in a vector that contains a gene that is involved in human disease of some sort.

其中一种应用就是,从动物,一般是老鼠体内取出受精卵,注入某种质粒,这种质粒带有与某些人类疾病相关的基因

耶鲁公开课 - 生物医学工程探索课程节选

You could do the same thing in mammals and here's one quick example of that, using a different kind of a vector, but inserting a gene into the fertilized egg of a mammal, in this case, a sheep.

你也能在哺乳动物上做到同样的效果,这里有另一个简短的例子,用了另一种载体,把一段基因注入哺乳动物的受精卵中,在这里我们用了绵羊

耶鲁公开课 - 生物医学工程探索课程节选

The first step is to take the DNA fragments that we're interested in and put them into this vector by basically cutting open the double stranded DNA and inserting the gene that we like in the region where we've cut.

第一步是把我们感兴趣的DNA片段,整合到这个载体里,基本的方法是切开质粒这个双链DNA,把我们想要的基因插入切开的区域

耶鲁公开课 - 生物医学工程探索课程节选