This is the website of the Restriction Enzyme Database.
这是限制酶数据库的网站。
Restriction enzyme site analysis was used to confirm the mutation.
突变位点经限制性内切酶分析证实。
Note: Underline sequences are cleavage sites of restriction enzyme.
注：标有下划线的碱基序列为限制酶切位点。
The recombinants were analyzed and identified by restriction enzyme, PCR and sequencing.
通过酶切、PCR及测序鉴定各重组体。
Both genes were correctly cloned and identified by PCR, restriction enzyme digestion and sequencing.
经pcr鉴定、酶切鉴定和测序说明所克隆的两种基因是正确的。
The length and the results of restriction enzyme digestion indicate that the amplified products are respected.
各扩增产物长度和限制性酶酶切结果表明均为预期产物。
Based on the number of restriction enzyme detecting RFLPs, most of mutations were attributed to point mutation.
根据揭示多态性的限制性内切酶的数量可将产生的突变大多归为点突变。
Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.
结果构建的EBO G87和EBO WT重组载体经内切酶双酶切鉴定及核苷酸序列测定证实。
The bovine, sheep and goat derived materials were positive in mixed feed powders of level test by PCR and restriction enzyme analysis.
PCR检测和酶切鉴定结果表明，在水平测试的混合饲料粉中可检出牛源、羊源性成分。
Restriction enzyme: Protein (more specifically, an endonuclease) produced by bacteria that cleaves DNA at specific sites along its length.
限制性内切：由细菌产生的一种蛋白质，能在特定的地方切断去氧核糖核酸分子。收藏。
Results:After being identified by PCR, restriction enzyme digestion and sequencing, the adeno-integrase hybrid system was successfully constructed.
结果：经PCR，酶切及测序方法鉴定，该腺病毒-整合酶嵌合系统构建成功。
Methods: 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction(PCR) technique.
方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（PCR）技术。
Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein.
结论：经酶切鉴定构建的人透明带蛋白3重组表达载体可高效表达重组人zp3蛋白。
Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.
结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。
Compared with AGE, CE is more outstanding in resolution and detection time, and it can be applied as a more effective means for DNA restriction enzyme pattern analysis.
结果表明，CE的分离效能明显高于age，是研究d NA限制性内切酶谱的更有效的检测手段。
Zinc finger nucleases (ZFNs) are artificial restriction enzymes made by fusing an engineered zinc finger DNA-binding domain to the DNA cleavage domain of a restriction enzyme.
锌指核酸酶是一种人工制做限制性内切酶，通过将锌指DNA结合区与限制性内切酶的DNA切割区融合获得。
When genome transplantations were performed using the restriction enzyme minus recipient cells, all the genome transplantations worked regardless of if the DNA was methylated or not.
结果显示不论DNA甲基化与否，所有使用受体细胞限制性内切酶的基因组移植都获得成功。
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
方法：应用聚合酶链反应（PCR）-双酶切法，对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。
CONCLUSION: Because of the speediness, simpleness and good specificity, the PCR combined with restriction enzyme digestion can be used as a primary screening in the gene diagnosis of CMT1A.
结论：由于PCR -双酶切方法快速、简单、易操作，且特异性好，可作为CMT1A基因诊断的一种初筛方法。
Plasmid DNAs were prepared by alkali lysis and purified with polyethylene glycol 8000. Restriction enzyme analysis show that both genes are well-constructed suitable for transgenic animal experiment.
限制性酶切图谱分析证实这两个基因的结构是完整的，符合转基因实验要求。
They are able to show that active peroxiredoxin 1, Prx1, an enzyme that breaks down harmful hydrogen peroxide in the cells, is required for caloric restriction to work effectively.
他们已经证明出活性过氧化物酶1（prx1），一种能够将细胞内有害的过氧化氢分解的酶类，正是此正面效应的所需酶类。
Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the AGAR gel electrophoresis.
最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。
We are able to show that caloric restriction slows down aging by preventing an enzyme, peroxiredoxin, from being inactivated.
我们已能够表明限制卡路里摄入能够通过阻断一种过氧化物酶的灭活而保持青春。
We are able to show that caloric restriction slows down aging by preventing an enzyme, peroxiredoxin, from being inactivated.
我们已能够表明限制卡路里摄入能够通过阻断一种过氧化物酶的灭活而保持青春。
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