Note: Underline sequences are cleavage sites of restriction enzyme.
注：标有下划线的碱基序列为限制酶切位点。
The recombinants were analyzed and identified by restriction enzyme, PCR and sequencing.
通过酶切、PCR及测序鉴定各重组体。
Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.
结果构建的EBO G87和EBO WT重组载体经内切酶双酶切鉴定及核苷酸序列测定证实。
The bovine, sheep and goat derived materials were positive in mixed feed powders of level test by PCR and restriction enzyme analysis.
PCR检测和酶切鉴定结果表明，在水平测试的混合饲料粉中可检出牛源、羊源性成分。
Results:After being identified by PCR, restriction enzyme digestion and sequencing, the adeno-integrase hybrid system was successfully constructed.
结果：经PCR，酶切及测序方法鉴定，该腺病毒-整合酶嵌合系统构建成功。
Methods: 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction(PCR) technique.
方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（PCR）技术。
Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein.
结论：经酶切鉴定构建的人透明带蛋白3重组表达载体可高效表达重组人zp3蛋白。
Zinc finger nucleases (ZFNs) are artificial restriction enzymes made by fusing an engineered zinc finger DNA-binding domain to the DNA cleavage domain of a restriction enzyme.
锌指核酸酶是一种人工制做限制性内切酶，通过将锌指DNA结合区与限制性内切酶的DNA切割区融合获得。
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
方法：应用聚合酶链反应（PCR）-双酶切法，对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。
CONCLUSION: Because of the speediness, simpleness and good specificity, the PCR combined with restriction enzyme digestion can be used as a primary screening in the gene diagnosis of CMT1A.
结论：由于PCR -双酶切方法快速、简单、易操作，且特异性好，可作为CMT1A基因诊断的一种初筛方法。
This is the website of the Restriction Enzyme Database.
这是限制酶数据库的网站。
Restriction enzyme site analysis was used to confirm the mutation.
突变位点经限制性内切酶分析证实。
Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.
结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。
Plasmid DNAs were prepared by alkali lysis and purified with polyethylene glycol 8000. Restriction enzyme analysis show that both genes are well-constructed suitable for transgenic animal experiment.
限制性酶切图谱分析证实这两个基因的结构是完整的，符合转基因实验要求。
Plasmid DNAs were prepared by alkali lysis and purified with polyethylene glycol 8000. Restriction enzyme analysis show that both genes are well-constructed suitable for transgenic animal experiment.
限制性酶切图谱分析证实这两个基因的结构是完整的，符合转基因实验要求。
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