Use of most molecular marker systems depends on the polymerase chain reaction (PCR), which is an important technique for amplifying specific DNA sequences.
大多数分子标记体系的利用都取决于聚合酶链反应（PCR），这是一项扩增特定DNA序列的重要技术。
The diagnosis is supported or confirmed by growing the bacteria from specimens of spinal fluid or blood, by agglutination tests or by polymerase chain reaction (PCR).
通过脊髓液或者血液标本培养出细菌，做凝集试验或者聚合酶链反应(PCR)实验，会支持或确认诊断结果。
Polymerase chain reaction-based (PCR-based) and enzyme-linked immunoabsorbent assay (ELISA) methods are now applied in the detection of major food-borne pathogens.
依据聚合酶链反应(PCR)和酶联免疫吸附检测(ELISA)的方法目前被应用于主要食源性致病菌的检测。
All had been evaluated using a slower but highly accurate test called real-time reverse transcription-polymerase chain reaction, or rRT-PCR, which checks for the genetic material of the virus.
应用一种缓慢但高度准确的试验，检测病毒的基因物质，叫即时逆转录-聚合酶链反应，或是rRT - P CR，全部做了评价。
Objective To study the value of multiplex polymerase chain reaction (PCR) in diagnosing herpes virus infection of eye bank cornea donor.
目的应用多引物pcr方法快速诊断眼库供体角膜疱疹病毒感染，探讨角膜移植术后移植衰竭和疱疹病毒感染的关系。
Objective:To evaluate the diagnostic value of polymerase chain reaction (PCR), culture, Semar acid fast staining, fluorescent staining in tuberculosis.
目的：评价聚合酶链反应（PCR） ，细菌培养，抗酸染色，荧光染色对结核病的诊断价值。
The HBV markers and HBV DNA in poultry sera, Yolk and bovine milk whey were detected by reversed passive hemagglutination assay (RPHA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR).
应用反向间接血凝试验（RPHA）、酶免疫测定（EIA）和聚合酶链反应（PCR）测定了家禽血清、卵黄和牛乳清中HBV标志物及人HBV-DNA。
OBJECTIVE To screen the primers and optimize the reaction systems for arbitrarily primed polymerase chain reaction(AP-PCR) on 8 sorts of bacteria.
目的筛选出最佳随机引物并优化其反应体系用于8种细菌的随机引物聚合酶链反应（AP PCR）分析。
Objective To investigate the fluorescence quantitative polymerase chain reaction (FQ-PCR) in the giant cell viral hepatitis in value.
目的探讨荧光定量聚合酶链反应(FQ - PCR)在巨细胞病毒性肝炎中的应用价值。
PCR the short name for "polymerase chain reaction," a sensitive lab test that can measure the presence of cancer cell-markers in the blood or marrow.
“聚合酶链式反应”的简称，一种可在血液或骨髓中检测到癌细胞标志物存在的灵敏的实验室试验。
The results of genotyping were compared with those obtained with the polymerase chain reaction method using sequence specific primers ( PCR-SSP).
并把分型结果与采用聚合酶链反应-序列特异性引物（PCR-SSP）获得的结果进行比较。
The polymerase chain reaction (PCR) machine is a basic instrument in molecule biology.
聚合酶链式反应仪(PcR仪)是分子生物学的基本仪器。
Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR) in detection of HCV RNA in serum and peripheral blood monocular cells (PBMC).
目的探讨实时荧光定量聚合酶链反应(FQ - PCR)在检测外周血清及单核细胞中hcvRNA含量的临床应用价值。
PCR-chip is a function biochip for DNA polymerase chain reaction. The temperature control section is so important that it decides the performance, dimension, integration of the whole chip.
P CR芯片是进行DNA聚合酶链式反应的芯片，温控部分的搭构是决定整个PCR芯片性能、尺寸、集成化的重要组成部分。
Methods We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) to examine 40 oral squamous cell carcinomas for loss of heterozygosity (LOH) at APC.
方法应用聚合酶链反应限制性片段长度多态性分析方法(PCR -RFLP)对40例口腔鳞癌组织中apc基因的杂合缺失(LOH)进行检测。
The principle, method and application of Polymerase Chain Reaction (PCR) was introduced in this paper.
介绍了分子生物学前沿技术聚合酶链式反应(PCR)的原理、方法及应用。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
A 40-base polymorphic repeat sequence located in the 3 '-untranslated region of the DAT gene was purified and amplified by polymerase chain reaction (PCR).
将位于多巴胺运输器基因3'端未转译区段的40 -碱基多形性重复序列予以纯化、经聚合酶链锁反应放大。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
方法：采用多重聚合酶链反应方法，以提取的基因组d NA为模板，在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
Objective to evaluate the clinicopathologic significance of the detection of peritoneal micrometastases in gastric cancer by reverse transcriptase-polymerase chain reaction (RT-PCR).
目的评估用逆转录聚合酶链式反应(RT PCR)方法检测胃癌腹腔微转移的临床病理意义。
Biochemical reaction, coagglutination test, metabolism inhibition test, polymerase chain reaction (PCR) assay, and DNA sequencing were employed to identify the isolated microorganisms.
阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA序列测定等方法进行鉴定。
Semiquantitative analysis of IL-6 in the hippocampus was done at different time and dose level after whole-brain irradiation with reverse-transcription polymerase chain reaction (RT-PCR).
应用逆转录聚合酶链反应(RT - PCR)半定量分析大鼠脑放射性损伤后海马区在不同时间、不同剂量水平IL - 6基因转录的动态表达。
Methods Using reverse transcription-polymerase chain reaction (RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
Methods the secretion specimen coming from the high risk patients with CA were examined with fluorescent quantitative polymerase chain reaction (FQ-PCR) for genotype HPV-DNA.
方法应用荧光定量聚合酶链反应(FQ - PCR)对尖锐湿疣高危人群分泌物标本进行HPV - DNA分型检测。
Objective To explore simple method for cloning the products amplified with polymerase chain reaction (PCR).
目的探讨克隆聚合酶链反应(PCR)扩增产物的简便方法。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
ObjectiveTo establish the rapid detection method of Enterotoxigenic E. coli(ETEC)by polymerase chain reaction(PCR)technology for characteristic primers.
目的利用PCR技术，尝试建立特异性引物PCR快速检测肠毒素大肠杆菌的方法。
Extron 1-5 of RHO gene was amplified by polymerase chain reaction (PCR), and the mutation of RHO gene was screened by direct DNA sequence measurement.
采用聚合酶链反应(PCR)方法扩增rho基因第1 ~ 5外显子和第1内含子基因片段，用直接dna测序法筛查rho基因突变。
Methods The length of genetic segment of SCA1, SCA2 and SCA3 in 92 patients with ataxia of unknown origin were detected by polymerase chain reaction (PCR) and agarose gel electrophoresis.
方法应用PCR方法和琼脂糖凝胶电泳对92例不明原因共济失调先证患者测定其SCA1、SCA2和SCA3基因片段长度。
Methods The length of genetic segment of SCA1, SCA2 and SCA3 in 92 patients with ataxia of unknown origin were detected by polymerase chain reaction (PCR) and agarose gel electrophoresis.
方法应用PCR方法和琼脂糖凝胶电泳对92例不明原因共济失调先证患者测定其SCA1、SCA2和SCA3基因片段长度。
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