As technological research findings will be industrialization, we choose the sucrose density gradient centrifugation as purified method and study the postorder work of purified virus-containing fluids.
鉴于产品开发要求，试验研究确定选用蔗糖密度梯度离心方法作为流感疫苗的纯化手段。
Methods Newcastle disease virus(NDV) was prepared in embryonic egg. NDV was purified by deposited of PEG-6000, speed centrifugation and sucrose—density gradient—centrifugation(SDGC).
方法利用传统鸡胚培养方法制备新城疫病毒（NDV），通过PEG-6000沉淀，差速离心和蔗糖密度梯度离心等方法提取纯化了新城疫病毒（NDV）。
Methods Newcastle disease virus(NDV) was prepared in embryonic egg. NDV was purified by deposited of PEG-6000, speed centrifugation and sucrose—density gradient—centrifugation(SDGC).
方法利用传统鸡胚培养方法制备新城疫病毒（NDV），通过PEG-6000沉淀，差速离心和蔗糖密度梯度离心等方法提取纯化了新城疫病毒（NDV）。
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