METHOD Whole-cell patch clamp technique was used.
方法采用全细胞膜片钳技术。
METHODS Using cell-attached patch clamp technique.
方法采用细胞贴附式膜片钳技术。
Methods Whole-cell patch clamp technique was used.
方法采用全细胞膜片钳制技术。
METHODS Whole cell recording by patch clamp technique.
方法膜片钳全细胞记录。
METHODS The whole cell patch clamp techniques were used.
方法全细胞膜片钳技术。
METHODS: Cell-attached configuration of patch clamp technique.
方法：细胞贴附式膜片钳技术。
Methods The whole-cell patch clamp with current clamp was used.
方法采用全细胞膜片钳技术，进行电流钳制。
METHODS The patch clamp whole cell recording technique was used.
方法采用膜片钳全细胞式记录技术。
Methods:Using nystatin perforated patch clamp recording configuration.
方法：采用制霉菌素穿孔膜片钳技术。
MethodsPerforated patch clamp techniques were employed on isolated rabbit SN cells.
方法运用穿孔膜片箝技术，在游离的兔心窦房结细胞上进行研究。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
方法：采用全细胞膜片钳技术，记录钠通道电流。
The widely using of patch clamp technique has greatly promoted our knowledge of ion channels.
膜片钳技术的广泛应用极大推动了人们对离子通道的认识。
Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
The change of sodium currents (INa) induced by U50,488H was detected by whole-cell recording mode using patch clamp.
常规酶解法分离成年大鼠心室肌细胞，采用全细胞膜片钳技术观察U50，488H对钠电流的作用。
CONCLUSION: the patch clamp technique makes it possible to observe the action sites of nicotine receptor antagonists.
结论：膜片钳技术使探讨烟碱受体拮抗药的作用位点成为可能。
Methods: By using patch clamp technique, measure voltage gated calcium currents in undifferentiated SH SY5Y cell line.
方法：通过膜片钳手段，测量未分化的SHSY5Y细胞钙离子通道电流；
This thesis discussed how to design and how to implement new software of patch clamp control and data acquisition system.
本文主要论述了新版本膜片钳系统软件的设计思路和实现方法。
The first step of recording calcium channel current of cardiomyocytes with patch clamp technique is dissociation of myocytes.
用膜片箝技术记录心肌细胞钙通道电流，首先必须获得单个心肌细胞。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
A moving patch clamp bath with a cell growth matrix coating layer relates to the patch clamp bath for electrophysiology experiment.
带细胞生长基质涂层的活动式膜片钳浴槽，它涉及一种电生理学实验用的膜片钳浴槽。
Methods: The isolated neurons of dorsal root ganglion (DRG) were studied using the whole cell patch clamp technique in current clamp mode.
方法：应用全细胞膜片箝电流箝制技术，对急性分离的大鼠DRG细胞进行电流箝制。
METHODS Voltage gated potassium outward current was recorded using whole cell patch clamp techniques in the cultured rat hippocampal neurons.
方法在培养的大鼠海马神经元上用膜片钳全细胞记录钾通道电流。
Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.
方法：运用全细胞膜片钳技术记录持续性钠电流，并观察其在缺氧-复氧模型下的变化。
A combination of mechanical and enzymatic isolation method is described for acutely isolating rat hippocampal neurons suitable for patch clamp recording.
结合酶消化和机械分离的方法，探讨一种适用于膜片箝记录的大白鼠海马神经元的急性分离技术。
In this paper we present an easily available method of intracellular dialysis via a microcatheter inserted into glass pipette during patch clamp experiment.
本文介绍了一种膜片钳微电极内插管进行胞内透析的方法。
The patch clamp recording techniques is an advanced electrophysiological technique, which has been widely used for research in the life science research field.
膜片钳技术是一种先进的电生理技术，在生命科学研究中已得到了广泛的应用。
Whole-cell patch clamp technique was used to study the electrophysiological characteristics of nociceptive trigeminal ganglion (TG) neurons labeled by nuclear yellow (NY).
实验利用全细胞膜片钳技术研究了荧光染料核黄（NY）标记的支配牙髓的痛感觉神经元的电生理特征。
In patch clamp experiment, the formation of giga-ohm seal is a critical step, which is usually fulfilled through controlling the air pressure within pipette and the holder.
在膜片钳实验中，吉欧封接的形成是至关重要的步骤，一般通过控制电极与电极夹持器内腔气体压力来完成。
Objective: to devise a method to isolate calcium-tolerant cardiomyocytes from SD rats for the experiment of patch clamp, and record the currents of L-type calcium channels.
目的：建立大鼠心肌细胞分离方法，用于膜片钳实验，并记录L型钙离子通道电流。
Method: the whole cell patch clamp recording technique was used in this study. Results: blood resin suppressed amplitude of the sodium channel currents in a dose dependent way.
方法：采用全细胞膜片钳记录法，在新鲜分离的大鼠背根神经节细胞上观察血竭对钠通道电流幅值的影响。
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