METHOD Whole-cell patch clamp technique was used.
方法采用全细胞膜片钳技术。
METHODS Using cell-attached patch clamp technique.
方法采用细胞贴附式膜片钳技术。
Methods Whole-cell patch clamp technique was used.
方法采用全细胞膜片钳制技术。
METHODS Whole cell recording by patch clamp technique.
方法膜片钳全细胞记录。
METHODS Patch clamp technique with whole cell configuration.
方法膜片钳制技术全细胞记录模式。
Methods: Patch clamp whole cell recording technique was used.
方法：膜片钳全细胞电流记录方法。
Methods The whole-cell patch clamp with current clamp was used.
方法采用全细胞膜片钳技术，进行电流钳制。
METHODS The patch clamp whole cell recording technique was used.
方法采用膜片钳全细胞式记录技术。
Methods:Using nystatin perforated patch clamp recording configuration.
方法：采用制霉菌素穿孔膜片钳技术。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
方法：采用全细胞膜片钳技术，记录钠通道电流。
The widely using of patch clamp technique has greatly promoted our knowledge of ion channels.
膜片钳技术的广泛应用极大推动了人们对离子通道的认识。
Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
The change of sodium currents (INa) induced by U50,488H was detected by whole-cell recording mode using patch clamp.
常规酶解法分离成年大鼠心室肌细胞，采用全细胞膜片钳技术观察U50，488H对钠电流的作用。
CONCLUSION: the patch clamp technique makes it possible to observe the action sites of nicotine receptor antagonists.
结论：膜片钳技术使探讨烟碱受体拮抗药的作用位点成为可能。
Methods: By using patch clamp technique, measure voltage gated calcium currents in undifferentiated SH SY5Y cell line.
方法：通过膜片钳手段，测量未分化的SHSY5Y细胞钙离子通道电流；
The first step of recording calcium channel current of cardiomyocytes with patch clamp technique is dissociation of myocytes.
用膜片箝技术记录心肌细胞钙通道电流，首先必须获得单个心肌细胞。
Conclusion a simple and fast method for acutely isolating rats cortex neurons suitable for patch clamp is successfully found.
结论成功建立了一种简单快速的适用于膜片钳技术的大鼠皮层神经元急性分离改良方法。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods the chloride channel currents were recorded in cultured A549 cells by using the whole-cell mode of patch clamp techniques.
方法在离体培养的A549细胞上，用膜片钳全细胞模式记录氯通道电流。
Methods: The isolated neurons of dorsal root ganglion (DRG) were studied using the whole cell patch clamp technique in current clamp mode.
方法：应用全细胞膜片箝电流箝制技术，对急性分离的大鼠DRG细胞进行电流箝制。
METHODS Voltage gated potassium outward current was recorded using whole cell patch clamp techniques in the cultured rat hippocampal neurons.
方法在培养的大鼠海马神经元上用膜片钳全细胞记录钾通道电流。
Because the differences of its application, the microelectrode can be divided into ion-selective microelectrode and patch clamp detecting technique.
根据检测目的的不同，微电极主要应用可以分为离子选择微电极检测技术和膜片钳技术。
Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.
方法：运用全细胞膜片钳技术记录持续性钠电流，并观察其在缺氧-复氧模型下的变化。
A combination of mechanical and enzymatic isolation method is described for acutely isolating rat hippocampal neurons suitable for patch clamp recording.
结合酶消化和机械分离的方法，探讨一种适用于膜片箝记录的大白鼠海马神经元的急性分离技术。
Patch clamp technique is a kind of technique which reflecting membrane molecular activity of one or more ion channel by record ion current of ion channel.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
The patch clamp recording techniques is an advanced electrophysiological technique, which has been widely used for research in the life science research field.
膜片钳技术是一种先进的电生理技术，在生命科学研究中已得到了广泛的应用。
Whole-cell patch clamp technique was used to study the electrophysiological characteristics of nociceptive trigeminal ganglion (TG) neurons labeled by nuclear yellow (NY).
实验利用全细胞膜片钳技术研究了荧光染料核黄（NY）标记的支配牙髓的痛感觉神经元的电生理特征。
In patch clamp experiment, the formation of giga-ohm seal is a critical step, which is usually fulfilled through controlling the air pressure within pipette and the holder.
在膜片钳实验中，吉欧封接的形成是至关重要的步骤，一般通过控制电极与电极夹持器内腔气体压力来完成。
In patch clamp experiments, for measuring the activities of live cells in various physiological environments, it is indispensable to perfuse a wide range of drugs to live cells.
膜片钳实验中，为了检测细胞在不同的生理环境下的生物活性，需要向活体细胞施加各种不同的药品。
Methods: The patch clamp was used to record the changes in average whole cell current and the conductance of GABA-A receptor on cultured hippocampal neurons in different developing stages.
方法用膜片钳记录仪在体外培养的不同时期，记录大鼠海马神经元GABA-A受体离子通道全细胞通道电流、电导的变化。
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