目的应用抑制性消减杂交技术构建人肾癌差异表达基因文库并进行差异表达基因筛选。
Objective To screen the genes differentially expressed in human renal cell carcinoma (RCC) cell lines using suppression subtractive hybridization (SSH).
目的：MCPR 1是我室利用消减杂交技术克隆出来的新基因，本研究利用融合蛋白制备mcpr1的多克隆抗体。
Objective: To prepare polyclonal antibodies of MCPR1, which is a novel Mouse Cleft Palate Relate gene cloned by subtractive hybridization method at our department.
结论DADS能诱导人白血病HL 6 0细胞分化，并引起相关的基因发生改变，而抑制性消减杂交技术能有效地分离差异表达的基因。
CONCLUSION DADS can induce human leukemia HL 60 cells differentiation, and suppression subtractive hybridization (SSH) technique is an effective method to isolate those differentially expressed genes.
方法：应用全反式维甲酸诱导前列腺癌DU 145细胞凋亡，建立细胞凋亡模型，采用基于PCR的改良消减杂交技术克隆与凋亡相关的基因。
Methods Improved PCR-based subtractive hybridization was used to clone the genes from apoptotic prostatic carcinoma cell line DU-145 cells induced by ATRA.
在这些技术当中，抑制消减杂交(SSH)具有快速、高效、假阳性率低等特点。
Among these techniques, the suppression subtractive hybridization (SSH) method has been shown to be quick and highly efficient, and it generates less false-positive clones.
利用消减抑制杂交技术建立对照组与实验组差异表达基因文库。
Differentially expressed gene librarys of the control and experimental groups were constructed by the method of suppression subtractive hybridization.
利用消减抑制杂交技术建立对照组与实验组差异表达基因文库。
Differentially expressed gene librarys of the control and experimental groups were constructed by the method of suppression subtractive hybridization.
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