免疫沉淀法纯化蛋白并用ERK1/2试剂盒测定ERK活性；
ERK activity was measured by ERK1/2 immuno-precipitation kinase assay kit;
从细胞萃取液或生物液体中分离酶的方法，基本上就是纯化蛋白质的方法。
The methods employed fOR enzyme isolation from cell extracts OR from biological fluids are essentially those fOR the purification of proteins.
研究者报道，NRF 2活性及其它的纯化蛋白和酶肿瘤标本中高于正常细胞。
NRF2 activity along with its cleansing proteins and enzymes were higher in tumor samples than normal cells, according to the researchers.
目的研究极性有机溶剂辅助阴、阳离子交换色谱串接组合纯化蛋白质的可行性。
PurposeTo explore the possibility of combination of anion and cation exchange chromatography by using the polarity organic solvents in eluent.
提纯的包涵体纯度可达70%以上，用镍亲和层析方法纯化蛋白则可达到95%。
The purity of inclusion bodies was above 70% and that of protein purified by nickel affinity chromatography was 95%.
为此，本研究建立了MALDI—TOFMS测试中在线纯化蛋白质样品的新方法。
A new method for on-line purification of samples with MALDI-TOFMS was established.
经SDSPAGE测定纯化蛋白的分子量，然后切下目的蛋白条带，进行蛋白质鉴定。
After determining the molecular weight of the purified protein by SDS PAGE, the band was excised from gel for protein identification.
以此纯化蛋白为靶，采用固体包被法对噬菌体展示随机十二肽库及环七肽库进行亲和筛选。
The purified protein coated on polystyrene plate was used as the target to carry out four rounds of bio-panning.
以免疫组化法用纯化蛋白制备的单克隆抗体分析NBEAL1表达与胶质瘤病理分级的相关性。
A NBEAL1 protein specific monoclonal antibody was prepared and was used to study the relationship of NBEAL1 expression with pathological grades of glioma.
目的克隆人热休克蛋白72 (HSP72)基因，原核表达并纯化蛋白产物，以探讨其生物学功能。
AIM To clone human heat shock protein 72 (HSP72) gene, do prokaryotic expression and purify HSP72 protein for further study.
该蛋白是从菌核病菌核菌中纯化得到的。
This protein has been purified from Sclerotinia sclerotiorum and the EPSP synthase has been analyzed.
将纯化的在大肠杆菌中表达的鹦鹉热衣原体（Cps）的重组主要外膜蛋白（MOMP）与油佐剂混合。
The purified recombinant major outer-membrane protein (MOMP) of Chlamydia psittaci (Cps) expressed in E. coli was mixed with oil adjuvant.
这病毒外部蛋白然后被提纯并产生几百到几千毫升的纯化病毒蛋白，作为抗原，是疫苗中的活性成分。
The outer proteins of the virus are then purified and the result is several hundred or thousand liters of purified virus protein that is referred to as antigen, the active ingredient in the vaccine.
抗体由蛋白A和肽段亲和层析纯化得到。
Antibodies are purified by protein A and peptide affinity chramotography.
多克隆抗体通过用DYKDDDDK合成肽免疫动物制备。抗体用蛋白a和多肽亲和色谱法纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein a and peptide affinity chromatography.
由于时间所限，拮抗蛋白的纯化、测序工作尚未完成，在以后的时间里，本实验室还将继续这方面的研究。
However, the purification of antimicrobial peptides have not yet been finished, it will be carried on in our laboratory in the future.
多克隆抗体由合成化肽段免疫动物，该合成肽段与YAP人源蛋白序列一致，抗体由蛋白a和肽段亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of YAP. Antibodies are purified by protein a and peptide affinity chromatography.
首先，我们在第11章中知道了关于大肠杆菌表达的蛋白产物的纯化工艺优化和特性研究。
First, we learn in Chapter 11 about process optimization and characterization studies for the purification of an E. coli-expressed protein product.
抗体采用蛋白a和肽亲和层析法纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
本文论述了基因重组融合蛋白纯化过程和存在的问题。
The paper describes process of recombinant fusion protein in purification and existing problems.
综述近年动物组织蛋白质的提取及纯化技术的研究进展，以期为进一步研究动物组织蛋白质提供帮助和参考。
The paper summarized the extracting and purifying technology of animal tissue protein in recent years, which provides the reference for the further research of animal tissue protein.
目的：表达和纯化半乳糖凝集素- 1融合蛋白。
Objective: To express and purify the fusion protein of galectin-1.
实验结果：(1)纯化的鞭毛蛋白经sDS - PAGE电泳，分子量约为65kd。
Results: (1) Molecular quantity of the purified flagellin was about 65kd by SDS-PAGE electrophoresis.
PAGE分析纯化过程中蛋白质组成的差异。
Analyse the protein difference of purification process Through SDS-PAGE.
中缅树?褐色脂肪组织解偶联蛋白质-1的纯化。
The purification of UCP-l(Uncoupling protein -1) from BAT(brown adipose tissue) of the tree shrew.
中缅树?褐色脂肪组织解偶联蛋白质-1的纯化。
The purification of UCP-l(Uncoupling protein -1) from BAT(brown adipose tissue) of the tree shrew.
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