In this study, a three factor and four level orthogonal experiment was designed to study the biologic effect of ultrasonic radiation on Marine Chlorella.

Method: Selecting the optimum carbon sources, nitrogen sources and inorganic salt to get the optimum liquid medium recipe by the orthogonal test in three factors and four levers;

This article introduces orthogonal design into clinical acupuncture and moxibustion and designs an orthogonal experiment with four factors and three levels to sift out the optimized plan.