The kit comprises the following main components: phosphoenol pyruvate carboxylase, pyruvate dehydrogenase and a stabilizer.

The types and the concentrations of different kinds of ion pair reagents, the contents of acetone and cyclohexane were considered as factors which affected the results of separation.

A series of color test are carried out to determine the influence of developing result at different time after preparing the acetylacetone on formaldehyde content measuring.