Methods: the expression of TLR4 and PCNA in cancer tissues and adjacent tissues were assessed by immunohistochemical method and the apoptosis was detected by the TUNEL method.
Methods The number of apoptosis cells were identified by TUNEL method and the expression intensity of antigen CD57 in lymph cells nearby the carcinoma was determined by S-P method.
3
方法采用免疫组化染色检测乳腺癌患者骨髓中肿瘤细胞的生物学标志及骨髓血浆的u PA活性。
Methods Immunocytochemical staining was used to study the biologic markers of disseminated tumor cells, and U-PA activity of the plasma in the bone marrow was assayed in breast cancer patients.