Characteristics of optical field in tapered optical fiber for dual-fiber optical trap is analyzed in this paper.

On this basis, then we discuss the other optical tweezers systems including Dual optical Trap tweezers, Hollow Laser tweezers, optic fiber Laser tweezers, Calculation Holography Laser tweezers.

Methods we constructed an optical tweezers which consists of a Laser, a microscope, a CCD camera and computer image processing system, and manipulated some cells with the tweezers.