内参基因

1
reference gene
关键词： 内参基因 ；实时定量 PCR；标准品；标准曲线 [gap=1169]Key words：reference genes；real time quantitative PCR；standards；standard curves
2
gapdh
...茎为处理,分别进行取样并提取RNA,反转录获得cDNA作模板,利用SYBR GreenⅠ成功构建了目的基因（GA20-oxidase）和内参基因（GAPDH）的标准品和标准曲线。
3
housekeeping gene
...：人、大鼠、小鼠、猪、牛、羊、鸡、斑马鱼、甘蔗等 • 样本处理与RNA提取 – cDNA – qPCR • 内参基因 ( housekeeping gene )选择 • 外界刺激– 10分钟– 可检测的表达改变 • 样品离开定义环境– 2分钟内–固定、裂解细胞 –RNA样本保存液 –Trizol –超...
4
internal control gene
... 关键词 内参基因 ； geNorm 程序；基因表达；实时定量 PCR；多酚氧化酶基因 [gap=11034]Key words internal control genes; geNorm program; gene expression; real-time qPCR; polyphenol oxidase gene.

短语

1
研究结果为鸭GAPDH作为内参基因用于鸭相关基因定量表达分析奠定了基础。
The results of this study will provide basis for GAPDH used as a reference gene to analyze the duck gene quantitatively.
2
目前基因表达的转录分析多采用单一看家基因作为内参来校正目标基因的表达量。
At present, transcription analysis of gene expression commonly USES a single housekeeping gene as control for normalization.
3
实验选用3-磷酸甘油醛脱氢酶基因（GAPDH）作为内参照，体外转录的RNA和阳性质粒作为两种标准品。
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was served as the internal reference, and RNA transcribed in vitro and positive plasmid were served as the standards in this experiment.

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