双抗体夹心法

1
double antibody sandwich method
双抗夹心法：一种用于检测特定分子的方法，其中两种抗体被用于夹持目标分子，以增强检测的灵敏度和特异性。

1
Double antibody sandwich method
双抗体夹心法（double antibody sandwich method,sandwich ELISA）是一种测定待测标本中是否含抗原的方法,步骤为：1,将含已知抗体的抗血清吸附在微量滴定板（microtiter plate ,聚...
2
double-antibody sandwich
...质芯片,双抗体夹心法,光学信号灰度值 [gap=1530]Key words】immun-gold staining; HCG; protein chip; double-antibody sandwich; intension of the staining IC spots ...
3
EILSA
(2)实验步骤：sIL-2R测定，采用双抗体夹心法(EILSA)，严格按照试剂盒要求操作。
4
Double Antibody Sandwich Enzyme-Link Immunoassay
关键词：儿童；CK-MB ；酶免疫抑制法；双抗体夹心法 [gap=1503]Key words: Child; CK-MB; Enzyme Inhibition Immunoassy; Double Antibody Sandwich Enzyme-Link Immunoassay

短语

1
采用酶法及酶联免疫双抗体夹心法测定血浆脂蛋白的浓度。
The concentrations of plasma lipoproteins were determined with enzyme and enzyme linked immunoassay double antibody methods.
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用免疫放射双抗体夹心法测定，标记抗体能保持良好的免疫活性。
The labelled antibody showed good immunoreactivity, when it was measured by IRMA-bi-antibody sandwich method.
3
结果：该双抗体夹心法，底物显色反应清楚肉眼即可作出判定，非特异干扰极小。
Results: The colored reaction of sandwich assay is very clear and easily judged by naked eyes.

双抗体夹心法
双抗体夹心法，是将含有已知抗体的抗血清吸附在微量滴定板上的小孔里，洗涤一次；加待测抗原，如两者是特异的，则发生结合，然后把多余抗体洗除；加入与待测抗原呈特异反应的酶联抗体，使形成“夹心”；加入该酶的底物，若看到有色的酶解产物产生，说明在孔壁上存在相应的抗原。

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