This paper introduced the principle, the targeting strategies, the screening mechanism and the commonly method in animals, plants and micro-organisms of gene knockout.
2
论述了基因敲除的基本原理、几种常用技术及该项技术在微生物育种方面的应用。
In this paper, the basic principles of gene knockout, several common techniques and their application in microbial breeding were introduced.
3
同时，课程也将对RNA干扰和基因敲除等重要的实验技术的原理及应用作专题介绍。
In addition, several useful laboratory techniques, e. g. small RNA interference and gene knockout are comprehensively introduced during the lecture.
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