Objective To evaluate the effect of 4 methods on acrylic resin base denture, namely, aseptic manipulation, steam cleaning, ultrasonic cleaning, and soaking in 2% glutaraldehyde solution.

Chitosan hydrogel was synthesized in acetic acid solution with chitosan using glutaraldehyde as crosslinker.

A waterborne multicolor paint is prepared by chitosan in acetic acid solution, mixing with short fiber and crosslinked by glutaric dialdehyde.