The result was Canvaxin, a vaccine against aggressive melanoma that was loaded up with 20 different tumor-specific components of melanoma, teaching the body new ways to recognize the disease.

The separation of tumor-infiltrating lymphocytes (TIL) from tumor mass of B16 melanoma was achieved by discontinuous density gradients centrifugation and the in vitro culture of TIL were investigated.

Using this device and the method of this invention can improve the width prepreg soakage property greatly.